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Delineation of signaling networks that underlie differences in macrophage phenotypic states

ABSTRACT: Quantitative proteomics and phosphoproteomics of primary human macrophages after exposure to different stimuli. Macrophages in the phenotypic states M1 (IFNγ + LPS), M2a (IL-4+IL-10), and M2c (IL-10) were characterized using global label-free LC-MS/MS quantification.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood, Macrophage

DISEASE(S): Disease Free

SUBMITTER: Jonas Bossart

LAB HEAD: Marija Buljan

PROVIDER: PXD043978 | Pride | 2025-11-24

REPOSITORIES: Pride

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			Action	DRS
	checksum.txt	Txt
	phosphoproteomics_Oxidation_M_Sites.txt	Txt
	phosphoproteomics_Phospho_STY_Sites.txt	Txt
	phosphoproteomics_RAW.zip	Other
	phosphoproteomics_allPeptides.txt	Txt

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Publications

Delineation of signaling routes that underlie differences in macrophage phenotypic states.

Totu Tiberiu T Bossart Jonas J Caire Elana E Sribike Katharina K Li Chen C Rottmar Markus M Sobottka Bettina B Yu Guocan G Ayala-Nunez Vanesa V Buljan Marija M

NAR molecular medicine 20250425 2

Macrophages represent a major immune cell type in tumor microenvironments, they exist in multiple functional states and are of strong interest for therapeutic reprogramming. While signaling cascades defining proinflammatory macrophages are better characterized, pathways that drive polarization in immunosuppressive macrophages are incompletely mapped. Here, we performed an in-depth characterization of signaling events in primary human macrophages in different functional states using mass spectrom ...[more]

PMID: 41255709

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