Proteomics

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Proteomic characterization of extracellular vesicles (EVs) released by primary human vascular smooth muscle cells (VSMCs) under calcification stress

ABSTRACT: Vascular calcification (VC) is a strong predictor of cardiovascular risk, particularly in chronic kidney disease (CKD) patients, associated with increased vascular stiffness, pulse pressure, left ventricular hypertrophy and atherosclerotic plaque burden [1]. VC is currently accepted as a highly controlled multifactorial process, where the release of extracellular vesicles (EVs) with calcification capacity plays an essential role in mediating cell-induced matrix mineralization [2]. Still, questions considering mechanisms of EVs deposition in the extracellular space, and its relationship with vesicle origin, loading, calcifying capacity and role in intercellular communication remain elusive. In this work we established a proteomic approach to characterize extracellular matrix (ECM)-deposited and cell media (CM) released EVs, from an in vitro model of VC consisting of primary vascular smooth muscle cells (VSMCs), bringing new knowledge into their specific characteristics and cargo.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Vascular Smooth Muscle Cell, Vascular Associated Smooth Muscle Cell

SUBMITTER: Teresa Mendes Maia  

LAB HEAD: Carla Viegas; Dina Simes

PROVIDER: PXD044045 | Pride | 2025-05-06

REPOSITORIES: Pride

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Publications

Gla Rich Protein (GRP) Mediates Vascular Smooth Muscle Cell (VSMC) Osteogenic Differentiation, Extracellular Vesicle (EV) Calcification Propensity, and Immunomodulatory Properties.

Viegas Carla C   Carreira Joana J   Maia Teresa M TM   Macedo Anjos L AL   Matos António P AP   Neves José J   Simes Dina D  

International journal of molecular sciences 20241119 22

Vascular calcification (VC) is a complex process involving vascular smooth muscle cell (VSMC) osteogenic differentiation, inflammation, and extracellular vesicle (EV) calcification and communication networks. Gla rich protein (GRP) is a calcification inhibitor involved in most of these processes. However, the molecular mechanism of GRP in VC and the specific characteristics, cargo, and functionality of calcifying EVs require further elucidation. Here, we use a combination of human ex vivo aortic  ...[more]

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