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Calculation of methylation of lysine residues of R2ab in the presence of nanoparticles

ABSTRACT: The R2ab was incubated with nanoparticles, then lysine residues were methylated and LC-MS/MS was performed to quantitate the extent of lysine methylation.

INSTRUMENT(S):

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Sandeep Misra

LAB HEAD: Sandeep Misra

PROVIDER: PXD044469 | Pride | 2025-09-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	R2ab-100nmPSNP-1.raw	Raw
	R2ab-100nmPSNP-1.xlsx	Xlsx
	R2ab-100nmPSNP-2.raw	Raw
	R2ab-100nmPSNP-2.xlsx	Xlsx
	R2ab-100nmPSNP-3.raw	Raw

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Publications

Exploring Residue-Level Interactions between the Biofilm-Driving R2ab Protein and Polystyrene Nanoparticles.

Somarathne Radha P RP Misra Sandeep K SK Kariyawasam Chathuri S CS Kessl Jacques J JJ Sharp Joshua S JS Fitzkee Nicholas C NC

Langmuir : the ACS journal of surfaces and colloids 20240104 2

In biological systems, proteins can bind to nanoparticles to form a "corona" of adsorbed molecules. The nanoparticle corona is of significant interest because it impacts an organism's response to a nanomaterial. Understanding the corona requires knowledge of protein structure, orientation, and dynamics at the surface. A residue-level mapping of protein behavior on nanoparticle surfaces is needed, but this mapping is difficult to obtain with traditional approaches. Here, we have investigated the ...[more]

PMID: 38174900

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