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Identification of small proteins in nodules of Robinia pseudoacacia

ABSTRACT: Robinia pseudoacacia L. (black locust) is capable of nitrogen fixation in association with rhizobia. Global transcriptional analysis in Robinia pseudoacacia nodules revealed the presence of three highly abundant small transcripts that may encode small proteins. The high number of proline and glycine residues is the most interesting feature of these proteins. A gelfree bottom up proteomics approach was used for the direct detection of the predicted small proteins. Crude protein extracts were obtained from nodules of wild Robinia (Robinia pseudoacacia). The endoproteases AspN and α-lytic protease were selected for protein digestion. One of these small proteins has been experimentally identified by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Robinia Pseudoacacia

TISSUE(S): Root Nodule

SUBMITTER: Martin Kucklick

LAB HEAD: Susanne Engelmann

PROVIDER: PXD044747 | Pride | 2026-03-06

REPOSITORIES: Pride

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			Action	DRS
	3_Prot_wo_signal_sequence_c.fasta	Fasta
	E10000_nodules_AspN_45_F7.raw	Raw
	E10001_nodules_AspN_80_F8.raw	Raw
	E10026_nodules_alpha_lytic_3_F1_4ul.raw	Raw
	E10027_nodules_alpha_lytic_6_F2.raw	Raw

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