Dataset Information

Proteomic profiling of plasma in patients with osteoarthritis and hemophilic arthropathy

ABSTRACT: Osteoarthritis (OA) and hemophilic arthropathy (HA) are clinically similar, but pathologically distinct conditions which result in joint pain and loss of function. Distinguishing their disease mechanisms is therefore a key step in the development of curative therapy, as opposed to current symptomatic treatments. Aim: To investigate the potential differences in plasma proteomic profile between OA and HA patients. Methods: This pilot cross-sectional study includes N=40 male participants equally divided across 4 subgroups: 1-2/haemophilia patients with severe or mild HA and 3-4/control subjects with severe or mild/no OA. We pooled plasma samples from each stage to search for biomarkers that accurately present each condition in order to achieve a biological sample which would represent a “biological average” as much as possible (1,2). The protein profile will be determined using liquid chromatography / mass spectrometry, after which functional gene enrichment analysis will be performed. Conclusion: The goal of the study is to uncover marked differences in plasma proteomic profile in patients with HA vs. OA which will potentially shed new light on the mechanisms of HA pathogenesis and could identify potential molecular therapeutic or diagnostic targets.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Václav Pustka

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD045469 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	5384_Exp1_108IS_DIA_75perc_25wind_1-1_1.raw	Raw
	5384_Exp1_108IS_DIA_75perc_25wind_1-2_1.raw	Raw
	5384_Exp1_108IS_DIA_75perc_25wind_1-3_1.raw	Raw
	5384_Exp1_108IS_DIA_75perc_25wind_1-4_1.raw	Raw
	5384_Exp1_108IS_DIA_75perc_25wind_1-5_1.raw	Raw

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Proteomic exploration of potential blood biomarkers in haemophilic arthropathy.

Kalebota Nataša N Novak Ruđer R Hrkač Stela S Perić Porin P Salai Grgur G Močibob Marko M Pranjić Marija M Zdráhal Zbyněk Z Pustka Václav V Žerjavić Nadica Laktašić NL Milošević Milan M Vodanović Marijo M Šalek Silva Zupančić SZ Grgurević Lovorka L

Health science reports 20240925 9

<h4>Background and aims</h4>The pathophysiology of haemophilic arthropathy (HA) is complex and largely undefined. Proteomic analyses provide insights into the intricate mechanisms of the HA.Our study aimed to identify differentially expressed proteins in relation to the severity of HA, explore their pathophysiological roles, and evaluate their potential as HA biomarkers.<h4>Methods</h4>Our cross-sectional observational study encompassed 30 HA patients and 15 healthy subjects. Plasma samples were ...[more]

PMID: 39323462

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