Dataset Information

Milk somatic cell proteomic profiling identifies potential biomarkers of host immune-related proteins regulating Bovine mastitis in tropical India's Sahiwal cows

ABSTRACT: Bovine mastitis remains a primary focus of dairy cattle disease research due to its considerable negative economic impact on the dairy industry. This was the first differential proteomic study aimed to identify milk somatic cell proteins contributing to host defense and their variable responses to naturally occurring mastitis in Sahiwal (Bos indicus) cows. To accomplish this, indigenous Sahiwal cows were categorized based on milk somatic cell counts (SCC)< 2×105cells/mL with no previous history of mastitis as healthy (H) animals. However, cows with sub-clinically mammary infection (SCM) and clinical mastitis (CM) had milk SCC 2-5×105 and > 5×105 cells/mL, respectively. The milk somatic cells were isolated and analysed using a label-free quantitative, liquid chromatography- mass spectrometry (LC-MS/MS) proteomic approach. In total, 3896, 3843 and 3868 proteins showed significant differential abundance between CM vs SCM, CM vs H and SCM vs H groups, respectively (=1 high-quality PSM/s, =1 unique peptide, FDR < 0.01), while 25, 53, 21 proteins were unique to CM, SCM and H groups, respectively. The differentially abundant proteins (DAPs) top significantly (p<0.05) upregulated proteins were annexin A5 (ANXA5), Rho-associated protein kinase 2 (ROCK2) and hepsin (HPN) in CM vs SCM groups, whereas vanin 2 (VNN2), thioredoxin reductase-like selenoprotein T (SELENOT), ceramidase (NAAA), lymphocyte antigen 75 (LY75/CD 205), cysteine-rich PDZ-binding protein (CRIPT), misshapen like kinase 1 (MINK1), macrophage scavenger receptor (MSR1/CD204), leupaxin (LPXN) and lipoamide acyltransferase (DBT) were upregulated common to both CM vs H and SCM vs H groups. These upregulated differentially abundant proteins (DAPs) are mainly associated with host immune functions of the mammary gland during mastitis. Besides this, the top downregulated proteins such as cortactin (CTTN), glutathione S-transferase (GSTM3), G protein-coupled receptor class C group 5 member B (GPRC5B), signal-induced proliferation-associated protein 1 (SPIA1), the exocyst complex component 6 (EXOC3), S100 A16. Additionally, the findings of the GO and KEGG enrichment functional analysis demonstrated Staphylococcus aureus infection, neutrophil extracellular trap formation, extracellular matrix receptor interaction, complement and coagulation cascades, toll-like and NOD-like receptor, PPAR, MAPK signalling, IL-17 signalling, Th1 and Th2 cell differentiation, Fc gamma mediated phagocytosis, cell adhesion molecules, peroxisome, lysosome, endocytosis were the enriched pathways of immune mechanisms regulating during naturally infected mastitis. These findings provide essential information on mammary infection-associated proteomic profiling in milk somatic cells and associated host immune response mechanisms which uncover potential biomarkers for detecting subclinical mastitis in dairy cattle.

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: LIJA S

LAB HEAD: Dr. Ajay Kumar Dang

PROVIDER: PXD045487 | Pride | 2025-05-06

REPOSITORIES: Pride

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Cytokine interactions and chemokine dysregulations in mastitis immunopathogenesis: insights from transcriptomic profiling of milk somatic cells in tropical Sahiwal (<i>Bos indicus</i>) cows.

Satheesan Lija L Dang Ajay Kumar AK Alex Rani R

Frontiers in immunology 20250324

<h4>Introduction</h4>Bovine mastitis causes a significant loss to the dairy industry by affecting the quantity and quality of milk. Addressing this challenge, the present study will leverage advanced omics techniques for early mastitis detection in early lactating Sahiwal cows (<i>Bos indicus</i>). This was the first differential transcriptomic study investigating the alterations in gene expression in milk somatic cells during the progression of naturally occurring mastitis in indigenous Sahiwal ...[more]

PMID: 40196120

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Project description:Bovine mastitis, the infection of the mammary gland which leads to great health and economic challenges for dairy farmers is accompanied by dramatic changes in the milk proteome. In this study of naturally occurring mastitis not only have the changes in the milk proteome been quantified in subclinical and clinical mastitis but simultaneous changes in the serum proteome have also been characterised and quantified. Milk and serum samples from healthy dairy cows (n=12) were compared to those of cows with subclinical (n=10) and clinical mastitis (n=112) using TMT label-based proteomic approach. The study included the milk and serum samples taken from thirty-two dairy cows ( kept on private farms located in Croatia. All cows were checked by physical examination. Somatic cells count (SCC) and mastitis test in milk samples were performed. According to the results, cows were assigned into three groups: Group I (control, n=10) consisted of healthy cows with SCC below 400,000 cells/ml on the monthly check-up and a negative mastitis test and without any clinical sign of mastitis. Group II (subclinical mastitis, n=12) comprised cows without clinical signs of mastitis but with SCC above 400,000 cells/ml on the monthly basis and a positive mastitis test at the time of sampling. Group III (clinical mastitis, n=10) consisted of cows with clinical signs of mastitis which include changes in milk appearance (flakes and clots in milk), different stages of udder inflammation (hyperemia, edema, pain, udder enlargement and elevated udder temperature) and disturbance of general health (depression, relaxed cold ears, dehydration, elevated body temperature, increased heart and respiratory rate, decreased ruminal contraction and decreased appetite). Blood samples were taken from v. coccygea and centrifuged at 3000 g for 15 min after clotting for two hours at room temperature. Serum samples were stored at -80°C until analysis. Milk samples were taken aseptically before the morning milking. First few streams were discarded. Teat ends were disinfected with cotton swabs soaked with 70% ethanol. Samples were taken into sterile tubes and transported to laboratory on ice within a few hours.

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Dataset Information

Milk somatic cell proteomic profiling identifies potential biomarkers of host immune-related proteins regulating Bovine mastitis in tropical India's Sahiwal cows

Dataset's files

Publications

Cytokine interactions and chemokine dysregulations in mastitis immunopathogenesis: insights from transcriptomic profiling of milk somatic cells in tropical Sahiwal (<i>Bos indicus</i>) cows.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Milk somatic cell proteomic profiling identifies potential biomarkers of host immune-related proteins regulating Bovine mastitis in tropical India's Sahiwal cows

Dataset's files

Publications

Cytokine interactions and chemokine dysregulations in mastitis immunopathogenesis: insights from transcriptomic profiling of milk somatic cells in tropical Sahiwal (&lt;i&gt;Bos indicus&lt;/i&gt;) cows.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Cytokine interactions and chemokine dysregulations in mastitis immunopathogenesis: insights from transcriptomic profiling of milk somatic cells in tropical Sahiwal (<i>Bos indicus</i>) cows.