Dataset Information

UV-A receptor CRY-DASH1 up- and UV-A receptor CRY-DASH1 up- and downregulates proteins involved in different plastidial pathways

ABSTRACT: Algae encode up to five different types of cryptochrome photoreceptors. So far, relatively little is known about the biological functions of the so-called DASH (Drosophila, Arabidopsis, Synechocystis and Homo)-type cryptochromes. The green alga Chlamydomonas reinhardtii encodes two of them. CRY-DASH1 also called DCRY1 has its maximal absorption peak in the UV-A range. It is localized in the chloroplast and plays an important role in balancing the photosynthetic machinery. Here, we performed a comparative analysis of chloroplast proteins from wild type and a knockout mutant of CRY-DASH1 named cry-dash1mut, using label-free quantitative proteomics as well as immunoblotting. Our results show upregulation of enzymes involved in specific pathways in the mutant including key enzymes of chlorophyll and carotenoid biosynthesis consistent with increased levels of photosynthetic pigments in cry-dash1mut. There is also an increase in certain redox as well as photosystem I and II proteins, including D1. Strikingly, CRY-DASH1 is coregulated in a D1 deletion mutant, where its amount is increased. In contrast, key proteins of the central carbon metabolism, including glycolysis/gluconeogenesis, dark fermentation and the oxidative pentose pathway are downregulated in cry-dash1mut. Similarly, enzymes of histidine biosynthesis are downregulated in cry-dash1mut leading to a reduction in the amount of free histidine. Yet, transcripts encoding for several of these proteins are at a similar level in the wild type and cry-dash1mut or even opposite. We show that CRY-DASH1 can bind to RNA, taking the the psbA RNA encoding D1 as target. These data suggest that CRY-DASH1 regulates plastidial metabolic pathways at the post-transcriptional level.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Chlamydomonas Reinhardtii Cc3269

TISSUE(S): Photosynthetic Cell

DISEASE(S): Disease Free

SUBMITTER: Volker Wagner

LAB HEAD: Maria Mittag

PROVIDER: PXD045811 | Pride | 2023-10-02

REPOSITORIES: Pride

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Publications

The UV-A Receptor CRY-DASH1 Up- and Downregulates Proteins Involved in Different Plastidial Pathways.

Rredhi Anxhela A Petersen Jan J Wagner Volker V Vuong Trang T Li Wenshuang W Li Wei W Schrader Laura L Mittag Maria M

Journal of molecular biology 20230910 5

Algae encode up to five different types of cryptochrome photoreceptors. So far, relatively little is known about the biological functions of the DASH (Drosophila, Arabidopsis, Synechocystis and Homo)-type cryptochromes. The green alga Chlamydomonas reinhardtii encodes two of them. CRY-DASH1 also called DCRY1 has its maximal absorption peak in the UV-A range. It is localized in the chloroplast and plays an important role in balancing the photosynthetic machinery. Here, we performed a comparative ...[more]

PMID: 37699454

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Dataset Information

UV-A receptor CRY-DASH1 up- and UV-A receptor CRY-DASH1 up- and downregulates proteins involved in different plastidial pathways

Publications

The UV-A Receptor CRY-DASH1 Up- and Downregulates Proteins Involved in Different Plastidial Pathways.

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