Dataset Information

HELA whole proteome of carboxypyridostatin (cPDS) treated cells

ABSTRACT: Cancer cells rely on mitochondria for their bioenergetic supply and macromolecule synthesis. Central to mitochondrial function is the regulation of mitochondrial protein synthesis, which primarily depends on the cytoplasmic translation of nuclear-encoded mitochondrial mRNAs whose protein products are imported into mitochondria. Despite the growing evidence that mitochondrial protein synthesis contributes to the onset and progression of cancer, and offers new opportunities for cancer therapy, knowledge of the underlying molecular mechanisms remains limited. Here, we show that RNA G-quadruplexes (RG4s) are co-localized with mitochondria and regulate mitochondrial function by modulating cytoplasmic mRNA translation of nuclear- encoded mitochondrial proteins. Our data support a model whereby the RG4 folding dynamics, under the control of oncogenic signaling and modulated by small molecule ligands or RG4-binding proteins, modifies mitochondria-localized cytoplasmic protein synthesis. Ultimately, this impairs mitochondrial functions, affecting energy metabolism and consequently cancer cell proliferation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Disease Free

SUBMITTER: Marjorie Leduc

LAB HEAD: Anne Cammas

PROVIDER: PXD046213 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AnCA220309__SB11_NT_SCX1_A47012_RK2_1_6837.d.7z	Other
	AnCA220309__SB11_NT_SCX2_A47013_RK3_1_6838.d.7z	Other
	AnCA220309__SB11_NT_SCX3_A47014_RK4_1_6839.d.7z	Other
	AnCA220309__SB11_NT_SCX4_A47015_RK5_1_6840.d.7z	Other
	AnCA220309__SB11_NT_SCX5_A47016_RK6_1_6841.d.7z	Other

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Publications

RNA G-quadruplexes control mitochondria-localized mRNA translation and energy metabolism.

Dumas Leïla L Shin Sauyeun S Rigaud Quentin Q Cargnello Marie M Hernández-Suárez Beatriz B Herviou Pauline P Saint-Laurent Nathalie N Leduc Marjorie M Le Gall Morgane M Monchaud David D Dassi Erik E Cammas Anne A Millevoi Stefania S

Nature communications 20250407 1

Cancer cells rely on mitochondria for their bioenergetic supply and macromolecule synthesis. Central to mitochondrial function is the regulation of mitochondrial protein synthesis, which primarily depends on the cytoplasmic translation of nuclear-encoded mitochondrial mRNAs whose protein products are imported into mitochondria. Despite the growing evidence that mitochondrial protein synthesis contributes to the onset and progression of cancer, and can thus offer new opportunities for cancer ther ...[more]

PMID: 40195294

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