Project description:Label free quantititative phosphoproteomics analysis following TiO2 enrichment, nanoscale capillary chromatography and high resolution tandem mass spectrometry.
Project description:Long non-coding RNA, LIRIL2R was identified and confirmed to be differentially expressed in regulatory T cells by RNAseq. A role of LIRIL2R was studied in iTreg cells using multiple approaches. Data independent analysis proteomics revealed effects of LIRIL2R knock down in Treg cells. Loss of Treg signature proteins upon LIRIL2R knock down signified the its role in iTreg cell development and function.
Project description:We studied the miRNA expression profile of heifers carrying male and female embryos in amniotic fluid and blood plasma at day 39 post-insemination, to determine the possible roles of miRNAs during the sex determination process.