Project description:We measured the impact of flotillin on the solubility of proteins after heat shock in Staphylococcus aureus, by measuring the effects of a knockout mutant.
Project description:Shigella is an intracellular bacterial pathogen known to activate numerous innate immunity and inflammatory signaling pathways. Many of these pathways are also involved in mTOR signaling. We used HEK293T cell cultures and sought to understand how Shigella infection and rapamycin treatment affect the transcriptome and in particular whether there were commonly affected pathways in the two experimental conditions. 8 samples were used - 2 control that were uninfected/untreated -- 1 sample lost due to RNA degradation, 3 infected with Shigella for 4 hours, and 3 treated with Rapamycin (50 ug/mL) for 4 hours. All samples were from independent experiments. We used the program AltAnalyze to perform pairwise comparisons between Uninfected with Infected, and Uninfected with Rapamycin using default parameters.
Project description:Background Compelling evidence indicates that Shigella species, the etiologic agents of bacillary dysentery, as well as enteroinvasive Escherichia coli, are derived from multiple origins of Escherichia coli and form a single pathovar. To further understand the genome diversity and virulence evolution of Shigella, comparative genomic hybridization microarray analysis was employed to compare the gene content of E. coli K-12 with those of 43 Shigella strains from all serotypes. Results For the 43 strains subjected to CGH microarray analyses, the common backbone of the Shigella genome was estimated to contain more than 1,900 open reading frames, with a mean number of 729 undetectable ORFs. The mosaic distribution of absent regions indicated that insertions and/or deletions have led to the highly diversified genomes of pathogenic strains. Conclusion These results support the hypothesis that by gain and loss of functions, Shigella species became successful human pathogens through convergent evolution from diverse genomic backgrounds. Moreover, we also found many specific differences between different lineages, providing a window into understanding bacterial speciation and taxonomic relationships. Keywords: comparative genomic hybridization
Project description:to analyse the transcriptomic response of human intestinal tissue engrafted in SCID mice to Shigella infection Keywords: infection, Shigella, gene expression, intestinal cell
Project description:We evaluated the transcriptome changes induced by infection of Hela 229 cells with Shigella flexneri. The sample set consists of a control (mock), total population of infected sample and infected sample sorted into Shigella positive and Shigella negative population.