Dataset Information

Trinucleotide cap analogs with N6-adenosine modifications: interaction partners in HEK293F cell line

ABSTRACT: A series of trinucleotide cap analogs functionalized with an amine-terminated linker at the guanosine ribose and immobilized on BrCN-activated Sepharose was prepared. These affinity resins AR-1 (Am), AR-2 (m6Am), and AR-3 (Bn6Am) were incubated with HEK293F cell extract in the presence of GTP to limit non-specific interactions. The pulled-down proteins were eluted with the corresponding trinucleotide cap analog (m7GpppAmpG for AR-1, m7Gpppm6AmpG for AR-2, and m7GpppBn6AmpG for AR-3), digested with trypsin, labeled with isobaric tags (TMT), and analyzed by shotgun proteomics. This experiment was performed in order to assess binding preferences of proteins to the resins AR-1, AR-2, and AR-3.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293f Cell

SUBMITTER: Remigiusz Serwa

LAB HEAD: Jacek Jemielity

PROVIDER: PXD046838 | Pride | 2024-03-08

REPOSITORIES: Pride

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Publications

Trinucleotide mRNA Cap Analogue N6-Benzylated at the Site of Posttranscriptional m6Am Mark Facilitates mRNA Purification and Confers Superior Translational Properties In Vitro and In Vivo.

Warminski Marcin M Trepkowska Edyta E Smietanski Miroslaw M Sikorski Pawel J PJ Baranowski Marek R MR Bednarczyk Marcelina M Kedzierska Hanna H Majewski Bartosz B Mamot Adam A Papiernik Diana D Popielec Agnieszka A Serwa Remigiusz A RA Shimanski Brittany A BA Sklepkiewicz Piotr P Sklucka Marta M Sokolowska Olga O Spiewla Tomasz T Toczydlowska-Socha Diana D Warminska Zofia Z Wolosewicz Karol K Zuberek Joanna J Mugridge Jeffrey S JS Nowis Dominika D Golab Jakub J Jemielity Jacek J Kowalska Joanna J

Journal of the American Chemical Society 20240305 12

Eukaryotic mRNAs undergo cotranscriptional 5'-end modification with a 7-methylguanosine cap. In higher eukaryotes, the cap carries additional methylations, such as m6Am─a common epitranscriptomic mark unique to the mRNA 5'-end. This modification is regulated by the Pcif1 methyltransferase and the FTO demethylase, but its biological function is still unknown. Here, we designed and synthesized a trinucleotide FTO-resistant N6-benzyl analogue of the m6Am ...[more]

PMID: 38442005

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Dataset Information

Trinucleotide cap analogs with N6-adenosine modifications: interaction partners in HEK293F cell line

Publications

Trinucleotide mRNA Cap Analogue <i>N</i>6-Benzylated at the Site of Posttranscriptional <sup>m6</sup>A<sub>m</sub> Mark Facilitates mRNA Purification and Confers Superior Translational Properties In Vitro and In Vivo.

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