Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adrenergic Signaling and Proteomic Responses in Breast Cancer Spheroids: Implications for Tumor Microenvironment and Therapeutic Strategies

ABSTRACT: Breast cancer, a global health challenge, exhibits molecular heterogeneity and complex interactions with the tumor microenvironment. We investigated adrenergic modulation in breast cancer spheroids, differentiating between basal-like (MDA-MB-231 and BT549) and luminal-like (T47D and MCF-7) subtypes. Noradrenalin reduced MDA-MB-231 spheroid size, reversed by propranolol, suggesting therapeutic potential. Luminal-like spheroids displayed subtler responses. Proteomic analysis revealed subtype-specific alterations, with TP53 as a consistent regulator affected by noradrenalin. Our study highlights subtype-dependent adrenergic signaling in breast cancer, providing insights for targeted therapies.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Ole Vidhammer Bjørnstad

LAB HEAD: Lars A. Akslen

PROVIDER: PXD046841 | Pride | 2025-10-13

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Fraction1.raw	Raw
	Fraction1_2.raw	Raw
	Fraction2.raw	Raw
	Fraction2_2.raw	Raw
	Fraction3.raw	Raw

Items per page:

1 - 5 of 19

Publications

Adrenergic signals influence proteomic responses in breast cancer cells.

Carrasco Manuel M Bjørnstad Ole Vidhammer OV Vethe Heidrun H Akslen Lars A LA

Frontiers in neuroscience 20250917

<h4>Introduction</h4>Breast cancer remains a major health challenge due to its molecular heterogeneity and complex interactions with the tumor microenvironment. Adrenergic signaling, mediated by stress hormones such as noradrenaline, has emerged as a potential regulator of cancer progression, influencing cell proliferation, cell adhesion, migration, and invasion.<h4>Methods</h4>This study investigates the effects of adrenergic modulation on breast cancer spheroids from basal-like (MDA-MB-231, BT ...[more]

PMID: 41040952

Similar Datasets

T cells facilitate brain metastasis of breast cancer.

Project description:Three breast cancer cell lines were co-cultured with T cells isolated from normal donors, in 3: 1 ratio. As controls, the breast cancer cells were cultured without T cells for three days. All cell cultures were washed three times with PBS to remove T cells before they were scraped and collected in 1.5 mL Eppendorf tubes. Three additional washing steps with PBS were applied. After removing the supernatant, the cell pellets were immediately snap-frozen on dry-ice and stored in -80 °C until the time of preparation. After thawing the samples, they were prepared and measured on nano LC.

2018-01-24 | PXD006750 | Pride

Microarray Analysis of Gene Expression in Human Normal and Breast Cancer Cells

Project description:Differential gene expression between normal breast epithelial cells and the malignant counterpart was studied using microarray technique. We utilized GeneAlbum GEM 1-6 from Incyte Pharmaceuticals, Inc., which contains which contains 65,873 cDNA clones (57,172 clones excluding controls), representing 33,515 individual genes. The normal human mammary epithelial cells (HMEC) from 3 donors were used to search different gene expression patterns among individuals, ages and races. In addition, 8 well-established breast cancer cell lines were used as probes against a common normal HMEC from 50 years old donor

2010-06-10 | E-GEOD-2155 | biostudies-arrayexpress

Identification of drug associated genes in ovarian cancer by RNA profiling

Project description:Two expression profilings were conducted in order to identify drug-associated genes in ovarian cancers. The first expression profiling was performed between RNAs from chemosensitive ovarian cancers and chemoresistant ovarian cancers. The second analysis was using a drug sensitive ovarian cancer cell line and a multi-drug resistant ovarian cancer cell line.

2010-06-30 | E-GEOD-2058 | biostudies-arrayexpress

T Lymphocytes induce the expression of Coronin-1A in breast cancer cells enabling the transmigration through a blood-brain-barrier model

Project description:Background: In previous work we discovered that T lymphocytes play a prominent role in the rise of brain metastases of ER-negative breast cancers. In the present study we explored expressional changes due to T cell contact associated with penetration through the BBB for breast cancer cell lines derived from cancers with various affinities for brain. Methods: Differential expression of proteins was identified by comparing the proteomes of the breast cancer cells before and after co-culture with T cells by using liquid chromatography-mass spectrometry (LC-MS). siRNA was used to silence protein expression in the tumor cells and the artificial BBB model was employed to study the effects on passage of the breast carcinoma cell lines. Results: Mass spectrometry-based proteomics revealed significant alterations in the expression of 35 proteins by the breast cancer cell lines upon T cell contact. Among the proteins is coronin-1A, a protein related to cell motility. Knockdown of CORO1A in the breast cancer cells reduced their ability to cross the artificial BBB to 60%. The effects were significantly less for the cell line derived from breast cancer with affinity for brain. The expression of coronin-1A was confirmed by immunohistochemistry and RT-PCR of 52 breast cancer samples of patients with metastasized breast cancers, with and without brain locations. Lastly, CORO1A upregulation was validated in a publicly available mRNA expression database from 204 primary breast cancers with known metastatic sites. Conclusions: We conclude that T lymphocytes trigger cancer cells to express proteins including coronin-1A thereby facilitating their passage through an in vitro BBB. In addition, a prominent role of coronin-1A in the formation of cerebral metastases in breast cancer patients is strongly suggestive by its upregulation in tissue samples of breast cancer patients with brain metastases.

2025-02-24 | PXD053787 | Pride

Global Lactylation Profiling Reveals Lactate-Driven Regulatory Mechanisms Promoting Tumor Progression in Oral Carcinoma

Project description:Cancer cells often rely on aerobic glycolysis for metabolism, and lactylation, a newly discovered post-translational modification, significantly impacts molecular processes. This study comprehensively analyzes lactylation's role in oral squamous cell carcinoma (OSCC), providing initial insights into its impact on progression. Six pairs of OSCC tissues underwent lactylation modification sequencing. 2,765 lactylation modification sites on 1,033 proteins were identified in OSCC, indicating its widespread presence. These modifications influenced metabolic activities, molecular synthesis, and transport.

2024-08-23 | PXD047673 | Pride

Proteomic profiling of colorectal adenomas

Project description:Colorectal adenomas are cancer precursor lesions of the large bowel. In these preinvasive lesions, a vast array of genomic and epigenomic changes have been detailed, but the consequence of these molecular alterations on the effectors of biological function (proteins) has not been comprehensively explored.

2014-04-03 | PXD000445 | Pride

Proteomic profiling of NCI60 cell lines from Cancer Cell Line Encyclopedia

Project description:This ArrayExpress record contains meta-data and results of quantitative analysis of cell lines from the NCI-60 panel using pressure cycling technology (PCT) and SWATH-mass spectrometry. Each cell line was analyzed in duplicate. Raw data files are available at the EMBL-EBI protemics data archive (PRIDE) at accession PXD003539 (http://www.ebi.ac.uk/pride/archive/projects/PXD003539). Since the record here does not include the raw data files and hence there is no need to explicitly link individual replicate to a raw file, each sample is only listed once in the ArrayExpress samples table for clarity.

2017-01-27 | E-PROT-2 | biostudies-arrayexpress

Promoter methylation in HNSCC tumor cell lines is significantly different from primary tumors and xenografts

Project description:This study focused on global methylation changes between tumors, normal mucosa, primary tumor xenografts, and cell lines in order to determine epigenetic changes in cell cultures and xenografts derived from primary tumors. 1 fully methylated control cell line, 1 fully demethylated control cell line, 5 HNSCC cell lines, 2 normal cell lines, 7 normal mucosa, 3 primary HNSCC tumors, 3 xenografts of primary tumors

2011-09-30 | E-GEOD-24787 | biostudies-arrayexpress

Pancreatic cells spectral library by the DIA strategy and the phenotype analysis of gemcitabine-sensitive

Project description:Data-independent acquisition (DIA)-mass spectrometry (MS) based proteome strategies is increasingly used for detecting and validating protein biomarkers and therapeutic targets. Here, by in-depth proteome analysis of 7 pancreatic cell lines, we built a pancreas-specific mass spectrum library containing 10633 protein groups and 184551 peptides.

2022-05-31 | PXD032263 | Pride

Protein and metabolic profiles of liver cancer cells

Project description:We established HCC cell lines resistant to these drugs and analyzed the correlation between protein and metabolite profiles using bioinformatics.

2024-05-20 | PXD052321 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data