Proteomics

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Illuminating the Function of the Orphan Transporter, SLC22A10 in Humans and Other Primates

ABSTRACT: HEK293 Flp-In cells were transfected transiently with various SLC22A10 orthologs, including human, chimpanzee, and the mutations to proline or leucine at position 220. After 72 hours of transfection, cell pellets were collected. The quantification was performed on both HEK293 cells and HEK293cells expressing the different SLC22A10 orthologs and mutations

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Merve Ceylan  

LAB HEAD: Per Artursson

PROVIDER: PXD047102 | Pride | 2024-04-09

REPOSITORIES: Pride

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SLC22A10 is classified as an orphan transporter with unknown substrates and function. Here we describe the discovery of the substrate specificity and functional characteristics of SLC22A10. The human SLC22A10 tagged with green fluorescent protein was found to be absent from the plasma membrane, in contrast to the SLC22A10 orthologs found in great apes. Estradiol-17β-glucuronide accumulated in cells expressing great ape SLC22A10 orthologs (over 4-fold, p<0.001). In contrast, human SLC22A10 displa  ...[more]

PMID: 37790518

Publication: 1/2

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