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Proteome changes induced by MPP8 gene knockout in SK-N-BE2 cells.

ABSTRACT: The alpha-herpesvirus Varicella-Zoster Virus (VZV), the causative agent of chicken pox, infects most humans worldwide and remains latent in peripheral neuronal cells for life. Its reactivation triggers herpes zoster and sporadic disseminated infection, in particular in the central nervous system (CNS) where it is associated with severe pathologies. VZV replication is efficiently controlled with antivirals, however the emergence of treatment-resistant VZV variants constitutes an increasing healthcare issue, calling for in-depth analysis of molecular VZV-host interactions to foster general knowledge and promote identification of novel therapeutic targets. Here we conducted a mass spectrometry-based survey in neuronal SK-N-BE2 cells, combining proteome analysis of VZV-infected cells with systematic characterization of VZV proteins’ functions through identifying their individual host partners and effects on the host proteome. We functionally evaluated the 116 most prominent host proteins by loss-of-function assay and unveiled yet uncharacterized dependency and restriction factors. In particular, the transcriptional regulators MPP8 and ZNF280D, interactors of VZV ORFs 4 and 12, and ORF63, respectively, were critical for VZV spread, thus highlighting these viral-host protein interactions as strategic targets to limit infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Virginie Girault

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD047393 | Pride | 2025-07-14

REPOSITORIES: Pride

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			Action	DRS
	20191125_QEp5_AnPi_SA_SKN_MPP8_P_02.raw	Raw
	20191125_QEp5_AnPi_SA_SKN_MPP8_P_03.raw	Raw
	20191125_QEp5_AnPi_SA_SKN_MPP8_P_04.raw	Raw
	20191125_QEp5_AnPi_SA_SKN_NTC_02.raw	Raw
	20191125_QEp5_AnPi_SA_SKN_NTC_03.raw	Raw

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