Proteomics

Dataset Information

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Affinity-purification of HA-tagged NPHP4 wild-type and S862N mutant in SK-N-BE2 cells


ABSTRACT: The alphaherpesvirus Varicella-Zoster Virus (VZV) infects most humans. It causes chickenpox, shingles and rare severe central nervous system (CNS) pathologies. To gain molecular insights in the virus’ pathobiology, we conducted a proteomic survey on the interactions and effects of 64 VZV proteins, and VZV infection-induced perturbations, in neuronal SK-N-BE2 cells. This identified 900 interactors and 3618 regulated host proteins (https://varizonet.innatelab.org). Data integration and functional validations of the identified host proteins unveiled NPHP4 as VZV restriction factor. Combining whole exome of patients with VZV-associated CNS pathologies identified the NPHP4 S862N variant. Importantly, the S862N mutation impaired NPHP4's antiviral activity, and supressed its interactions with 14-3-3 proteins, as measured by affinity-purification.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Virginie Girault  

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD061602 | Pride | 2025-07-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
GFP.fasta Fasta
HF1_0130_V0016-01_S001620_L_LFQ_01.raw Raw
HF1_0130_V0016-01_S001621_L_LFQ_01.raw Raw
HF1_0130_V0016-01_S001622_L_LFQ_01.raw Raw
HF1_0130_V0016-01_S001623_L_LFQ_01.raw Raw
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