Affinity-purification of HA-tagged NPHP4 wild-type and S862N mutant in SK-N-BE2 cells
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ABSTRACT: The alphaherpesvirus Varicella-Zoster Virus (VZV) infects most humans. It causes chickenpox, shingles and rare severe central nervous system (CNS) pathologies. To gain molecular insights in the virus’ pathobiology, we conducted a proteomic survey on the interactions and effects of 64 VZV proteins, and VZV infection-induced perturbations, in neuronal SK-N-BE2 cells. This identified 900 interactors and 3618 regulated host proteins (https://varizonet.innatelab.org). Data integration and functional validations of the identified host proteins unveiled NPHP4 as VZV restriction factor. Combining whole exome of patients with VZV-associated CNS pathologies identified the NPHP4 S862N variant. Importantly, the S862N mutation impaired NPHP4's antiviral activity, and supressed its interactions with 14-3-3 proteins, as measured by affinity-purification.
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER:
Virginie Girault
LAB HEAD: Andreas Pichlmair
PROVIDER: PXD061602 | Pride | 2025-07-14
REPOSITORIES: Pride
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