Proteomics

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SILAC experiment of HEK 293 cells upon RPS26 silencing

ABSTRACT: In recent years, there is emerging pool of evidence suggesting that ribosomal composition plays a role in selection of transcripts that are actively translated at given time and tissue. Some ribosomal subunits are not the core components of 40S ribosome and alternatively can be assembled or disassembled from 40S subunit. For example, a 40S ribosomal protein - RPS26 is one of such alternative ribosomal subunits. In ordered to investigate what proteins are under the RPS26-depended translation, we performed stable isotope labeling using amino acids in cell culture (SILAC) experiment 48 hours post siRNA-based RPS26 silencing in HEK293 cells and revealed the pool of proteins that are up or downregulated upon RPS26 depletion.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Anna Baud  

LAB HEAD: Krzysztof Sobczak

PROVIDER: PXD047397 | Pride | 2025-05-26

REPOSITORIES: Pride

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20230908_Anna_Baud_HEKcell_SILAC_mix-L4H4_1.raw Raw
20230908_Anna_Baud_HEKcell_SILAC_mix-L4H4_2.raw Raw
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Publications

Insufficiency of 40S ribosomal proteins, RPS26 and RPS25, negatively affects biosynthesis of polyglycine-containing proteins in fragile-X associated conditions.

Tutak Katarzyna K   Broniarek Izabela I   Zielezinski Andrzej A   Niewiadomska Daria D   Skrzypczak Tomasz T   Baud Anna A   Sobczak Krzysztof K  

eLife 20250516

Expansion of CGG repeats (CGGexp) in the 5' untranslated region (5'UTR) of the <i>FMR1</i> gene underlies the fragile X premutation-associated conditions including tremor/ataxia syndrome, a late-onset neurodegenerative disease and fragile X-associated primary ovarian insufficiency. One common pathomechanism of these conditions is the repeat-associated non-AUG-initiated (RAN) translation of CGG repeats of mutant <i>FMR1</i> mRNA, resulting in production of FMRpolyG, a toxic protein containing lon  ...[more]

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