Proteomics

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Label-free proteomics analysis of plasma membrane and tonoplast fractions of elicited grapevine cell cultures.

ABSTRACT: In this study, grapevine cells control and elicited with 50 mM methylated cyclodextrins and 0.1 mM methyl jasmonate, that are known to accumulate extracellular resveratrol, were used to obtain membrane enriched fractions, specifically tonoplast and plasma membrane enriched fractions with the aim of finding candidate transporters of resveratrol. Label-free proteomic experiment was carried out using triplicates of the tonoplast and the plasma membrane enriched fractions. Protein identification was performed at the PROTEORED© proteomic facility of the University of Alicante (Spain) as detailed in the sample processing section.

INSTRUMENT(S):

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Plant Cell, Cell Suspension Culture

SUBMITTER: Roque Bru-Martinez  

LAB HEAD: Roque Bru Martinez

PROVIDER: PXD048454 | Pride | 2025-05-06

REPOSITORIES: Pride

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Publications

The grapevine ABC transporter B family member 15 is a <i>trans</i>-resveratrol transporter out of grapevine cells.

Martínez-Márquez Ascensión A   Martins Viviana V   Sellés-Marchart Susana S   Gerós Hernâni H   Corchete Purificación P   Bru-Martínez Roque R  

Frontiers in plant science 20250120

Stilbenes, particularly <i>trans</i>-resveratrol, play a highly relevant defense role in grapevines as phytoalexin is induced in response to stress. Metabolism and transport of stilbenes can be conveniently investigated in grapevine cell culture since large amounts of <i>trans</i>-resveratrol are accumulated in the extracellular medium upon treatment with the elicitor methylated cyclodextrin, either alone or combined with methyl jasmonate. A proteomic approach on grapevine cell membrane fraction  ...[more]

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