Proteomics

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FTIR, XPol, and LC-MS confirm endogenous collagen in Edmontosaurus fossil bone C4PR_LIV

ABSTRACT: Reports of proteins in fossilized bones have ignited an on-going debate because previously it was generally assumed that fossilization results in destruction of all organic components. We combine Attenuated Total Reflection (ATR) Fourier Transform InfraRed (FTIR) spectroscopy, cross-polarized light microscopy (XPol), and two variations of Liquid Chromatography Mass Spectrometry (LC-MS), to detect and verify endogenous collagen protein in fossilized bone. Data from an Edmontosaurus dinosaur excavated from Upper Cretaceous strata of the South Dakota Hell Creek Formation were compared with that from thermally decayed modern turkey (Meleagris) bone. The four methods show different evidence for collagen presence in the fossilized bone. ATR-FTIR spectra show a signal for carbonyl (C=O) groups, a repeat moiety in protein amino acid sequences. XPol shows the birefringence of collagen within Meleagris, as well as Edmontosaurus. LC-MS bottom-up proteomics showed m/z agreement between multiple ions from trypsin-digested samples and identified peptide sequences thus far only identified in a hadrosaur. Tandem LC-MS unambiguously identified hydroxyproline, a unique collagen-indicator amino acid, in acid-digested samples from the same.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine) Dinosauria Meleagris Gallopavo (common Turkey)

SUBMITTER: Philip Brownridge  

LAB HEAD: Stephen Taylor

PROVIDER: PXD048810 | Pride | 2025-03-24

REPOSITORIES: Pride

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Dataset's files

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Action DRS
260423_Bovine_Collagen_1hr_1_in_1000.mgf Mgf
260423_Bovine_Collagen_1hr_1_in_1000.mzid.gz Mzid
260423_Bovine_Collagen_1hr_1_in_1000.raw Raw
260423_Turkey_Collagen_1hr_1_in_100.mgf Mgf
260423_Turkey_Collagen_1hr_1_in_100.mzid.gz Mzid
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Publications

Evidence for Endogenous Collagen in <i>Edmontosaurus</i> Fossil Bone.

Tuinstra Lucien L   Thomas Brian B   Robinson Steven S   Pawlak Krzysztof K   Elezi Gazmend G   Faull Kym Francis KF   Taylor Stephen S  

Analytical chemistry 20250117 5

Reports of proteins in fossilized bones have been a subject of controversy in the scientific literature because it is assumed that fossilization results in the destruction of all organic components. In this paper, a novel combination of analytical techniques is used to address this question for an exceptionally well-preserved <i>Edmontosaurus</i> sacrum excavated from the Upper Cretaceous strata of the South Dakota Hell Creek Formation. Cross-polarized light microscopy (XPol) shows birefringence  ...[more]

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