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COL1A2 Barcoding: Bone Species Identification via Shotgun Proteomics

ABSTRACT: Species identification of fragmentary bones remains a challenging task in archeology and forensics. A species identification method for such fragmentary bones that has recently attracted interest is the use of bone collagen proteins. We developed a method similar to DNA barcoding that reads collagen protein sequences in bone and automatically determines the species by performing sequence database searches. We tested our method using bone samples from 30 vertebrate species ranging from mammals to fish.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Thunnus Maccoyii Lithobates Catesbeianus Takifugu Rubripes Columba Livia Numida Meleagris Sus Scrofa Domesticus (domestic Pig) Struthio Camelus (ostrich) Ovis Aries Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri) Phasianus Colchicus Salmo Salar (atlantic Salmon) Oreochromis Niloticus Rattus Norvegicus (rat) Coturnix Japonica Equus Caballus (horse) Xiphias Gladius Ursus Arctos (brown Bear) (grizzly Bear) Paralichthys Olivaceus Seriola Dumerili Cervus Elaphus (red Deer) Bos Taurus (bovine) Cyprinus Carpio (common Carp) Gallus Gallus (chicken) Thunnus Albacares Oryctolagus Cuniculus (rabbit) Anas Platyrhynchos Pelodiscus Sinensis Capra Hircus (goat) Crocodylus Porosus Mus Musculus (mouse)

TISSUE(S): Bone

SUBMITTER: Kei Taniguchi

LAB HEAD: Hajime Miyaguchi

PROVIDER: PXD045402 | Pride | 2023-12-14

REPOSITORIES: Pride

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