Dataset Information

Proteomic Analysis of Tear Fluid from the Offspring of Keratoconus Patients Toward Novel Biomarkers and Pathways Associated with Corneal Biomechanical Weakness and Disease Susceptibility.

ABSTRACT: Keratoconus (KC) is a chronic and degenerative condition marked by pathological weakening, thinning, and protrusion of the corneal tissue. Corneal biomechanical weakness constitutes a hallmark of early keratoconus (KC) stages, which has underlined the importance of the study of biomechanical response in the early clinical screening of KC. In this line, the family history of KC is consistently identified as the leading risk factor for KC development, and the first-order pediatric relatives of KC patients are considered the most vulnerable population for KC development. On the other hand, tear fluid has become an attractive and accessible source that could be useful in identifying the molecular targets of several ocular diseases, such as corneal ectasias and more specifically KC disease. So, based on the vulnerability of first-order pediatric KC relatives to develop the disease, as well as the potential of tear fluid as a biomarker source, the purpose of this study was to analyze quantitatively and qualitatively the tear proteome of young KC offspring using micro–liquid chromatography coupled to tandem mass spectrometry, trying to get closer to the molecular drivers underlying the alterations in corneal biomechanical properties at at-risk stages of KC and to provide new insights into the molecular mechanisms involved in the earlier stages of the disease.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tear

DISEASE(S): Keratoconus

SUBMITTER: Susana Bravo

LAB HEAD: Susana Belén Bravo López

PROVIDER: PXD049296 | Pride | 2025-06-09

REPOSITORIES: Pride

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Tear Proteomic Analysis From Offspring of Keratoconus Patients: New Insights Into Corneal Biomechanical Weakness and Disease Risk Stages.

López-López Maite M Regueiro Uxía U Bravo Susana S Pena Carmen C Pastoriza Yaiza Y Conde-Amboage Mercedes M Hervella Pablo P Lema Isabel I

Investigative ophthalmology & visual science 20250501 5

<h4>Purpose</h4>To analyze the tear proteome of keratoconus offspring (O-KC) and assess the molecular drivers underlying corneal biomechanical weakening at KC-risk stages.<h4>Methods</h4>This cross-sectional study included 80 O-KC young participants and 42 controls without a KC family history. Based on the corneal biomechanical behavior, O-KC eyes were classified as low, moderate, and high-risk of KC development (O-KC-LR, O-KC-MR, O-KC-HR). Tear fluid was extracted using Schirmer strips, and the ...[more]

PMID: 40434347

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			Action	DRS
	O_KC_HR_1.wiff	Wiff
	O_KC_HR_1.wiff.scan	Wiff
	O_KC_HR_10.wiff	Wiff
	O_KC_HR_10.wiff.scan	Wiff
	O_KC_HR_11.wiff	Wiff