Effects of different nanoplastics on macrophages in a repeated or single exposure mode

ABSTRACT: Plastics are one of the most preoccupying emerging pollutants. Macroplastics released in the environment degrade into microplastics and nanoplastics. Because of their small size, these micro and nano plastic particles can enter the food chain and, in addition to their ecotoxicological effects, contaminate humans with still poorly known biological effects. Plastics being particulate pollutants, they are handled in the human body by scavenger cells such as macrophages, which are important players in the immune system. One of the major issues with plastic contamination of living cells is their extremely variable biodegradability. Many petroleum-based plastics such as polyethylene and polystyrene are very poorly biodegradable, while the degradability of other plastic types such as polyesters is very variable. Moreover, the exposure of humans to plastics is typically a chronic, repeated exposure, and not an acute one as in most of the in vitro toxicology studies devoted to nanoplastics.In this research project, we compare the proteomes of macrophages exposed to repeated doses (8x10µg/ml) of different plastic particles, in order to decipher their responses to these pollutants of concern. We included in this study two poorly biodegradable plastics (polystyrene and poly-ethyleneterephthalate) and two biodegradable plastics (polylactide and polycaprolactone). For comparisons reasons, the data obtained on cells exposed to the same cumulative dose at once (1x80µg/ml) are also included.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Disease Free

SUBMITTER: Hélène Diemer  

LAB HEAD: Sarah Cianferani

PROVIDER: PXD049997 | Pride | 2026-02-09

REPOSITORIES: Pride