Comparison of Cerium nanoparticles toxicity against Bacillus subtilis grown in two different types of biofilm
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ABSTRACT: The project is focused on the soil bacterium model Bacillus subtilis that is one of the essential components of the rhizosphere. In its natural ecosystem, this bacterium develops in the form of a biofilm, particularly around plant roots. Two types of biofilm that are closer to its natural environment than the usual liquid type culture, have been assayed, pellicle biofilm and swarming. We then used the power of shotgun proteomics and bioinformatics tools to analyze in details these two somehow similar modes of cultures. Several physiological and metabolic patterns including proteins implicated in sporulation have shown significant differences triggered by the growth culture mode. The effect of two types of nanoparticles SiO2 and the widely used nanoceria (cerium oxide nanoparticles) has then been studied in the same way. This allowed us to investigate in details the response of the bacteria to the cerium oxide nanoparticles and especially to focus on the differences observed depending on the biofilm type, including proteins associated with iron homeostasis. This clearly highlighted the importance of eventually testing several growth conditions and pinpointed the power of proteomics helping building mechanistic hypotheses.
INSTRUMENT(S):
ORGANISM(S): Bacillus Subtilis
TISSUE(S): Cell Culture
SUBMITTER:
Hélène Diemer
LAB HEAD: Sarah CIANFERANI
PROVIDER: PXD055248 | Pride | 2026-02-09
REPOSITORIES: Pride
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