Carrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering

ABSTRACT: We added increasing amounts of E coli carrier proteome to BSA, our moch recombinant protein to model interference of high-background BSA peaks with smaller peaks of E coli HCPs, using MS2 and MS3 methods to show co-fragmentation and co-elution based on similar m/z ratio. We created an interference detection channel to solve the problem of interference.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Divyanshi Karmani  

LAB HEAD: John Patrick Murphy

PROVIDER: PXD050183 | Pride | 2026-01-12

REPOSITORIES: Pride