Proteomics

Dataset Information

0

Discovery, target identification and lead optimisation of a quinazoline series with potent anti-Chagasic activity


ABSTRACT: We next used an approach to determine the direct binding of quinazolines to their target(s). A protein can be stabilised or destabilised through the binding of a ligand, altering that proteins thermostability. Such alterations in thermostability can cause a ligated protein to denature at a different temperature from its unligated counterpart (Corpas-Lopez and Wyllie, 2021; Paradela et al., 2021; Lima et al., 2022; Milne et al., 2022; Hanna et al., 2023). Isothermal proteome profiling (IPP), which detects changes in protein thermostability following the addition of drug to a lysate, was used to determine which proteins from the entire T. cruzi proteome bind to Q371 and Bhambra-10.3.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Trypanosoma Cruzi Dm28c

SUBMITTER: Victoriano Corpas-Lopez  

LAB HEAD: Susan Wyllie

PROVIDER: PXD050235 | Pride | 2025-04-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
367_2022-VCL-TcC-Q371b.raw Raw
367_2022-VCL-TcT-Q371a.raw Raw
367_2022-VCL-TcT-Q371b.raw Raw
367_2022_VCL-TcC-Q371a.raw Raw
Bhambra48-aC48.raw Raw
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