Proteomics

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: Functional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (PTP4A1 silencing and IFN treatment of NCI-H1944 cells)

ABSTRACT: The subcellular localization of proteins can be highly dynamic and regulated by post-translational modifications. Farnesylation is a lipid modification that enables anchoring of proteins to cellular membranes, and thus, spatially resolved signaling and activity. Due to the importance of farnesylation for cellular functions, farnesyltransferase inhibitors (FTIs) including tipifarnib were developed and are being tested in oncology clinical trials. The current lack of knowledge on the functional impact of FTIs on hundreds of farnesylation substrates limits response prediction and their use in cancer precision medicine. Here, we report a global functional proteomics investigation of farnesylation in multiple lung cancer cell lines. In this dataset, we reveal farnesylated protein PTP4A1 as a regulator of interferon signaling using global proteomics data on PTP4A1-silenced and/or interferon gamma-stimulated NCI-H1944 cells.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Lung Cancer

SUBMITTER: Georgios Mermelekas  

LAB HEAD: Lukas Orre

PROVIDER: PXD050446 | Pride | 2025-05-13

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
FTI_MS_rawfile_list_v240307.xlsx Xlsx
H1944_PTP4A1si_IFN_genes_table.txt Txt
Olena_20230508_FTI_H1944_TMT16_Set1_400ug_IPG3-10_fr02.raw Raw
Olena_20230508_FTI_H1944_TMT16_Set1_400ug_IPG3-10_fr03.raw Raw
Olena_20230508_FTI_H1944_TMT16_Set1_400ug_IPG3-10_fr04.raw Raw
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Functional-proteomics-based investigation of the cellular response to farnesyltransferase inhibition in lung cancer.

Pan Yanbo Y   Berkovska Olena O   Marathe Soumitra S   Mermelekas Georgios G   Gudoityte Greta G   Wolide Amare D AD   Arslan Taner T   Seashore-Ludlow Brinton B   Lehtiö Janne J   Orre Lukas M LM  

iScience 20250121 2

Farnesylation is a lipid post-translational modification of proteins crucial for protein membrane anchoring and cellular signaling. Farnesyltransferase inhibitors (FTIs), such as tipifarnib, are being tested in cancer therapy. However, the full impact of FTIs on farnesylation substrates remains poorly understood, thus limiting their use in precision medicine. In this study, we performed a global proteomics analysis to investigate farnesylation and the effects of tipifarnib in lung cancer cell li  ...[more]

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