Dataset Information

Mlf Mediates Proteotoxic Response via Formation of Cellular Foci for Protein Folding and Degradation

ABSTRACT: Myeloid leukemia factor 1 (Mlf1) was identified as a proto-oncoprotein that affects hematopoietic differentiation in humans. However, its cellular function remains elusive, spanning roles from cell cycle regulation to modulation of protein aggregate formation and participation in ciliogenesis. Given that structurally conserved homologs of Mlf1 can be found across the eukaryotic tree of life, we decided to characterize its cellular role underlying this phenotypic pleiotropy. Using a model of the unicellular eukaryote Giardia intestinalis, we demonstrate that its Mlf1 homolog (GiMlf) mainly localizes to two types of cytosolic foci: microtubule nucleation zones, where it interacts with Hsp40, and ubiquitin-rich, membraneless compartments, found adjacent to mitochondrion-related organelles known as mitosomes, containing the 26S proteasome regulatory subunit 4. Upon cellular stress, GiMlf either relocates to the affected compartment or disperses across the cytoplasm, subsequently accumulating into enlarged foci during the recovery phase. In vitro assays suggest that GiMlf can be recruited to membranes through its affinity for signaling phospholipids. Importantly, cytosolic foci diminish in the gimlf knockout strain, which exhibits extensive proteomic changes indicative of compromised proteostasis. Consistent with data from other cellular systems, we propose that Mlf acts in the response to proteotoxic stress by mediating the formation of function-specific foci for protein folding and degradation.

INSTRUMENT(S):

ORGANISM(S): Giardia Intestinalis (giardia Lamblia)

SUBMITTER: Pavel Talacko

LAB HEAD: Pavel Doležal

PROVIDER: PXD050586 | Pride | 2025-05-13

REPOSITORIES: Pride

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Mlf mediates proteotoxic response via formation of cellular foci for protein folding and degradation in Giardia.

Vinopalová Martina M Arbonová Lenka L Füssy Zoltán Z Dohnálek Vít V Samad Abdul A Bílý Tomáš T Vancová Marie M Doležal Pavel P

PLoS pathogens 20241021 10

Myeloid leukemia factor 1 (Mlf1) was identified as a proto-oncoprotein that affects hematopoietic differentiation in humans. However, its cellular function remains elusive, spanning roles from cell cycle regulation to modulation of protein aggregate formation and participation in ciliogenesis. Given that structurally conserved homologs of Mlf1 can be found across the eukaryotic tree of life, we decided to characterize its cellular role underlying this phenotypic pleiotropy. Using a model of the ...[more]

PMID: 39432513

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