Dataset Information

Molecular mechanisms of target organ damage in youth with primary hypertension

ABSTRACT: High blood pressure or Primary Hypertension is associated with target organ damage (TOD) in both adults and youth, where TOD predicts the adverse cardiovscular (CV) events. The design and methods of the SHIP AHOY (Study of High Blood Pressure in Pediatrics, Adult Hypertension Onset in Youth) project have been discussed elsewhere (Mendizabal B, et al., 2018, Hypertension. 2018 Sep;72(3):625-631.). Briefly, SHIP AHOY study participants with normal (Cut off: SBP <80th%), and high- systolic blood pressure (Cut off: SBP >90th%), were stratified as with or without TOD, like Low LVMI (Left ventricular mass index) (Cut off: <38.6 gm/m2.7), and High LVMI (Cut off: >38.6 gm/m2.7), were selected in the present study cohort. Blood samples (for PBNCs and serum) were collected to investigate genetic, epigenetic, and proteomics changes that influence the development of hypertensive TOD in youth. We excluded youth with symptomatic severe hypertension, on antihypertensive or lipid-lowering medication in the past 6 months, with diabetes mellitus (type 1 or 2), kidney disease, or other chronic medical conditions. All study participants and their parents provided written informed consent and assent according to local investigational review board requirements. The mechanisms involved in blood pressure mediated TOD are limitedly understood in onset of TOD. To understand this, using RNA sequencing, we first identified candidate differentially expressed genes in circulating PBMCs of study participants. Next, through miRNA and whole genome bisulphite sequencing of circulating PBMCs we determined the epigenetic regulators of TOD. Also, using LCMS, serum proteomics, the key circulatory protein involved in advancing CV- TOD events was identified. In conclusion, multiomics approach was utilized to define the circulatory regulators involved in BP mediated CV-TOD.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Qin Fu

LAB HEAD: Qin Fu

PROVIDER: PXD050915 | Pride | 2025-12-15

REPOSITORIES: Pride

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Source:

			Action	DRS
	BeckerArif10422287Set1.mgf	Mgf
	BeckerArif10422287Set1.msf	Msf
	BeckerArif10422287Set1_1.raw	Raw
	BeckerArif10422287Set1_2.raw	Raw
	BeckerArif10422287Set1_3.raw	Raw

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Publications

A Multiomics Approach to Defining Target-Organ Injury in Youths With Primary Hypertension: The SHIP AHOY Cohort.

Ananthamohan Kalyani K Brady Tammy M TM Arif Mohammed M Daniels Stephen R SR Falkner Bonita B Ferguson Michael M Flynn Joseph T JT Hanevold Coral C Hooper Stephen R SR Ingelfinger Julie R JR Lande Marc M Martin Lisa J LJ Meyers Kevin E KE Mitsnefes Mark M Rosner Bernard B Samuels Joshua A JA Kuffel Gina G Zilliox Michael J MJ Chen Qin M QM Becker Richard C RC Urbina Elaine M EM Sadayappan Sakthivel S

Journal of the American Heart Association 20251028 21

<h4>Background</h4>Primary hypertension in childhood tracks into adulthood and is associated with increased cardiovascular risk. Studies conducted in individuals aged <18 years, an age group without many of the confounding comorbid cardiovascular disease risk factors in adults, provide an opportunity to explore early cardiovascular target-organ injury.<h4>Methods</h4>Youths (n=132, mean age, 15.8 years) were stratified by blood pressure (BP) as low-BP, mid-BP, and high-BP and by left ventricular ...[more]

PMID: 41147417

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