LC-MS/MS of thoracic aorta and perivascular adipose tissue following short-term dietary methionine restriction

ABSTRACT: We are exploring pathways influenced by short-term dietary methionine restriction (MR) in thoracic aorta and associated perivascular adipose tissue (PVAT) from obese C57BL/6J male mice around 26 weeks of age. Mice were fed 60% high fat diet (HFD) until they were obese, experimental mice were given 60% HFD with 0.12% methionine for 3, 5, and 10 days while the control group maintained a 60% HFD with 0.86% methionine. All mice were euthanized on the same day, then thoracic aorta was harvested, the PVAT was removed, and both sample types were prepared and analyzed separately. Three mice per group were assessed with LC-MS/MS. MR diet induced lean phenotype in PVAT, which was observed histologically, and we hypothesized that changes in lipid metabolism and mitochondrial bioenergetics mediated this response.

INSTRUMENT(S):

ORGANISM(S): Arachis Hypogaea (peanut)

TISSUE(S): Blood Plasma, Early Embryonic Cell

DISEASE(S): Polycystic Kidney

SUBMITTER: Carlos Gartner  

LAB HEAD: Marissa McGilvrey

PROVIDER: PXD051504 | Pride | 2025-04-07

REPOSITORIES: Pride