Molecular characterization of ribosome defective cell lines
Ontology highlight
ABSTRACT: We generated a unique panel of isogenic cell models with the following genotypes via CRISPR-Cas9 engineering: Rpl5+/-, Rpl10 R98S, Rpl11+/-, Rpl22+/-, Rpl22-/-, Rps15 P131S and Rps15 H137Y. As cellular background, we chose for Ba/F3 cells. These are mouse lymphoid pro-B-cells, offering a relevant cellular background as most of these mutations have been linked to lymphoid malignancies as T-ALL and CLL. Quantitative proteomics profiling of this isogenic cell line panel shed a light on the molecular biology underlying the oncogenic mechanisms of these ribosomal lesions.
INSTRUMENT(S):
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Cell Culture, Hematopoietic Cell
SUBMITTER:
Delphi Van Haver
LAB HEAD: Kim De Keersmaecker
PROVIDER: PXD051831 | Pride | 2025-11-18
REPOSITORIES: Pride
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