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Mass-spectrometry analysis of vehicle or AKB-9778 treated FOXF2KO and WT human endothelial cells

ABSTRACT: To validate our results on Foxf2 related Tie2 signaling and explore their transferability to human cells, we studied human iPSC-derived endothelial cells (iECs). FOXF2KO and WT iECs (González-Gallego et al., in preparation) were treated with AKB-9778 or vehicle and assessed by proteomics. Similar to our results in mouse BECs, proteomic analysis revealed a dysregulation of multiple members of the TIE2 signaling pathway in vehicle treated FOXF2KO compared to WT iECs including TIE2 and NOS3. Moreover, treatment with AKB-9778 restored the levels of multiple TIE2 signaling related proteins. Collectively, these findings demonstrate that AKB-9778 rescues the deficiency of TIE2 signaling in human endothelial cells lacking FOXF2. González-Gallego, J. et al. A fully iPSC-derived 3D model of the human blood-brain-barrier for exploring neurovascular disease mechanisms and therapeutic interventions - in preparation, available from editorial office upon request.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pluripotent Stem Cell, Endothelial Cell Of Vascular Tree

DISEASE(S): Brain Small Vessel Disease

SUBMITTER: Stephan Mueller

LAB HEAD: Stefan F.

PROVIDER: PXD051855 | Pride | 2025-09-02

REPOSITORIES: Pride

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			Action	DRS
	KO_RAZ_r2_1_Slot2-29_1_4349.d.zip	Other
	KO_RAZ_r2_2_Slot2-45_1_4368.d.zip	Other
	KO_RAZ_r2_3_Slot2-17_1_4337.d.zip	Other
	KO_RAZ_r2_4_Slot2-40_1_4363.d.zip	Other
	KO_VEH_r2_1_Slot2-24_1_4344.d.zip	Other

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