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Pax6os1 pull-down in MIN6 cell lysate

ABSTRACT: Pull-down experiment to identify protein binding partners of the lncRNA Pax6os1 in mouse insulinoma cell line MIN6 using in-vitro transcribed RNA.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Type B Pancreatic Cell, Cell Culture

SUBMITTER: Timothy Pullen

LAB HEAD: Guy Rutter

PROVIDER: PXD052133 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	Rebecca_Callingham_010319_Double_SILAC_Set_1_PS.msf	Msf
	Rebecca_Callingham_010319_Double_SILAC_Set_2_PS.msf	Msf
	Set1_Slice_1.raw	Raw
	Set1_Slice_2.raw	Raw
	Set1_Slice_3.raw	Raw

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Publications

Roles for the long non-coding RNA Pax6os1/PAX6-AS1 in pancreatic beta cell function.

Lopez-Noriega Livia L Callingham Rebecca R Martinez-Sánchez Aida A Nawaz Sameena S Pizza Grazia G Haberman Nejc N Cvetesic Nevena N Nguyen-Tu Marie-Sophie MS Lenhard Boris B Marchetti Piero P Piemonti Lorenzo L de Koning Eelco E Shapiro A M James AMJ Johnson Paul R PR Leclerc Isabelle I Hastoy Benoit B Gauthier Benoit R BR Pullen Timothy J TJ Rutter Guy A GA

iScience 20241209 1

Long non-coding RNAs (lncRNAs) are emerging as crucial regulators of beta cell function. Here, we show that an lncRNA-transcribed antisense to Pax6, annotated as Pax6os1/PAX6-AS1, was upregulated by high glucose concentrations in human as well as murine beta cell lines and islets. Elevated expression was also observed in islets from mice on a high-fat diet and patients with type 2 diabetes. Silencing Pax6os1/PAX6-AS1 in MIN6 or EndoC-βH1 cells increased several beta cell signature ...[more]

PMID: 39811653

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