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Characterisation of TACC3 affimer interactions by HDX-MS

ABSTRACT: Here we characterise the interactions of affimers against TACC3 by HDX-MS.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Antonio Calabrese

LAB HEAD: Antonio Calabrese

PROVIDER: PXD052409 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	240202_HDX_fbsaca_09.raw.zip	Raw
	240202_HDX_fbsaca_10.raw.zip	Raw
	MR_240203.DnX	Other
	fbsaca_HDX_240203_01.raw.zip	Raw
	fbsaca_HDX_240203_03.raw.zip	Raw

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Publications

Structural characterization and inhibition of the interaction between ch-TOG and TACC3.

Shelford James J Burgess Selena G SG Rostkova Elena E Richards Mark W MW Larocque Gabrielle G Sampson Josephina J Tiede Christian C Fielding Alistair J AJ Daviter Tina T Tomlinson Darren C DC Calabrese Antonio N AN Pfuhl Mark M Bayliss Richard R Royle Stephen J SJ

The Journal of cell biology 20250319 6

The mitotic spindle is a bipolar array of microtubules, radiating from the poles which each contain a centrosome, embedded in pericentriolar material. Two proteins, ch-TOG and TACC3, have multiple functions at the mitotic spindle due to operating either alone, together, or in complex with other proteins. To distinguish these activities, we need new molecular tools to dissect their function. Here, we present the structure of the α-helical bundle domain of ch-TOG that mediates its interaction with ...[more]

PMID: 40105698

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