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Bladder cancer cell-derived exosomes LC-MSMS

ABSTRACT: To detect the differential expressed proteins in bladder cancer(BCa)-derived exosomes, we isolated BCa cell-derived exosome and normal bladder epithelial cell-derived exosomes. The quantitative proteomics were conducted to detect the upregulated proteins in BCa cell-derived exosomes compared with SV-HUC-1-derived exosomes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: YAN LIN

LAB HEAD: Changhao Chen

PROVIDER: PXD053106 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

Integrin α6-containing extracellular vesicles promote lymphatic remodelling for pre-metastatic niche formation in lymph nodes via interplay with CD151.

Lin Yan Y Zheng Hanhao H Jia Linpei L Luo Yuming Y Zhang Dingwen D An Mingjie M Pang Mingrui M Diao Xiayao X Li Wenjie W Chen Jiancheng J Li Yuanlong Y Liu Daiyin D Liu Zhicong Z Huang Jian J Lin Tianxin T Chen Changhao C

Journal of extracellular vesicles 20241001 10

Heterogeneous extracellular vesicles (EVs) from various types of tumours are acknowledged for inducing the formation of pre-metastatic "niches" in draining lymph nodes (LNs) to promote lymphatic metastasis. In order to identify the specific subpopulations of EVs involved, we performed high-resolution proteomic analysis combined with nanoflow cytometry of bladder cancer (BCa) tissue-derived EVs to identify a novel subset of tumour-derived EVs that contain integrin α6 (ITGA6<sup>+</sup>EVs) and re ...[more]

PMID: 39329462

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2025-05-07 | PXD052771 | Pride

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