Dataset Information

Protein Extraction methods suitable for muscle tissue proteomic analysis

ABSTRACT: • Evaluation of two methods for protein extraction from muscle tissues. • Two different extraction methods showed distinct patterns of protein abundance; • Two-dimensional electrophoresis revealed 131 spots with different volumes; • SDS-based buffer (method A) mostly extracted proteins of high MW range and peculiar in sarcomere and muscular fibres, such as Troponin, Myosin, Miozenin 2 and Ankyrin; • Urea/chaps/DTE/tris solution (method B) enriched numerous protein species in a low MW range, several protein fragments and organelle membrane proteins

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Soleus

SUBMITTER: Lorenza Vantaggiato

LAB HEAD: LUCA BINI

PROVIDER: PXD053117 | Pride | 2025-05-07

REPOSITORIES: Pride

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Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis.

Vantaggiato Lorenza L Landi Claudia C Shaba Enxhi E Rossi Daniela D Sorrentino Vincenzo V Bini Luca L

Proteomes 20240925 4

Muscle tissue is one of the most dynamic and plastic tissues of the mammalian body and covers different roles, such as force generation and metabolic control. Muscular proteomics provides an important opportunity to reveal the molecular mechanisms behind muscle pathophysiology. To ensure successful proteomic analysis, it is necessary to have an efficient and reproducible protein extraction method. This study aimed to evaluate the efficacy of two different extraction protocols of muscle samples f ...[more]

PMID: 39449499

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