Dataset Information

The Tel1 Phosphoproteomic Network

ABSTRACT: Large-scale unbiased phosphoproteomics analysis of Tel1-deleted yeast reveal dozens of novel Tel1-dependent phosphorylation events. The canonical S/T-Q motif is enriched amongst these Tel1-dependent sites, but a novel D/E-S/T motif is even more highly enriched. Phosphoproteomics of possible downstream acidophilic kinases Dun1, Rad53, and Hrr25 are performed to determine if these sites may be indirectly Tel1-dependent, but the D/E-S/T sites are not dependent on any of them.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: William Comstock

LAB HEAD: Marcus Smolka

PROVIDER: PXD053305 | Pride | 2025-05-07

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	091520_WJC_Hrr25i_VS_150_1pct.mzXML	Mzxml
	091520_WJC_Hrr25i_VS_150_1pct.pep.xml	Pepxml
	091520_WJC_Hrr25i_VS_150_1pct.raw	Raw
	091620_WJC_Hrr25i_VS_150_MMS_F01.mzXML	Mzxml
	091620_WJC_Hrr25i_VS_150_MMS_F01.pep.xml	Pepxml

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Publications

Profiling Tel1 signaling reveals a non-canonical motif targeting DNA repair and telomere control machineries.

Comstock William J WJ Bhattarai Shrijan S Sanford Ethan J EJ Navarro Marcos V A S MVAS Smolka Marcus B MB

The Journal of biological chemistry 20250116 3

The stability of the genome relies on phosphatidyl inositol 3-kinase-related kinases (PIKKs) that sense DNA damage and trigger elaborate downstream signaling responses. In Saccharomyces cerevisiae, the Tel1 kinase (ortholog of human ATM) is activated at DNA double-strand breaks (DSBs) and short telomeres. Despite the well-established roles of Tel1 in the control of telomere maintenance, suppression of chromosomal rearrangements, activation of cell cycle checkpoints, and repair of DSBs, the subst ...[more]

PMID: 39826692

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