Dataset Information

Hemicellulose utilisation in Flavobacterium

ABSTRACT: The plant hemicellulose xyloglucan (XyG) is secreted from the roots of numerous plant species, including cereals, and contributes towards soil aggregate formation in terrestrial systems. Whether XyG represents a key nutrient for plant-associated bacteria is unclear. The phylum Bacteroidota are abundant in the plant microbiome and provide several beneficial functions for their host. However, the metabolic and genomic traits underpinning their success remain poorly understood. Here, we employed whole-cell proteomics to determine the molecular mechanisms responsible for xyloglucan utilisation in two model Flavobacterium species, Flavobacterium johnsoniae DSM2064 and Flavobacterium sp. OSR005. We identified the occurrence of a distinct and conserved gene cluster, referred to as the Xyloglucan Utilisation Loci (XyGUL). Flavobacterium XyGUL is a hybrid of the molecular machinery found in gut Bacteroides spp., Cellvibrio japonicus, and the plant pathogen Xanthomonas. Combining protein biochemistry, computational modelling and phylogenetics, we identified a mutation in the enzyme required for initiating hydrolysis of the XyG polysaccharide, an outer membrane endoxyloglucanase glycoside hydrolase family 5 subfamily 4 (GH5_4), which enhances activity towards XyG.

INSTRUMENT(S):

ORGANISM(S): Flavobacterium Sp. F52

SUBMITTER: Andrew Bottrill

LAB HEAD: Dr Ian Lidbury

PROVIDER: PXD053370 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	TT_220216_16_MS22-025_2064_GLU1_Slot1-16_1_2286.d.zip	Other
	TT_220216_16_MS22-025_2064_GLU1_Slot1-16_1_2286.mzid.gz	Mzid
	TT_220216_16_MS22-025_2064_GLU1_Slot1-16_1_2286.mzid_TT_220216_16_MS22-025_2064_GLU1_Slot1-16_1_2286.MGF	Mzid
	TT_220216_16_MS22-025_2064_GLU2_Slot1-17_1_2287.d.zip	Other
	TT_220216_16_MS22-025_2064_GLU2_Slot1-17_1_2287.mzid.gz	Mzid

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Publications

Metabolism of hemicelluloses by root-associated Bacteroidota species.

Martin Hannah H Rogers Lucy A LA Moushtaq Laila L Brindley Amanda A AA Forbes Polly P Quinton Amy R AR Murphy Andrew R J ARJ Hipperson Helen H Daniell Tim J TJ Ndeh Didier D Amsbury Sam S Hitchcock Andrew A Lidbury Ian D E A IDEA

The ISME journal 20250101 1

Bacteroidota species are enriched in the plant microbiome and provide several beneficial functions for their host, including disease suppression. Determining the mechanisms that enable bacteroidota to colonise plant roots may therefore provide opportunities for enhancing crop production through microbiome engineering. By focusing on nutrient acquisition mechanisms, we discovered Bacteroidota species lack high affinity ATP-binding cassette transporters common in other plant-associated bacteria fo ...[more]

PMID: 39913342

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