Dataset Information

Proteome changes in response to water deficit in developing maize ovules

ABSTRACT: This project aims to identify changes in the proteome of maize ovules that precede their abortion in response to water deficit. To take into account variations along development, the proteome was analyzed in ovules collected at different positions along the ear at two stages of ear development. Plants were grown in a greenhouse and watered up to tassel emergence. Irrigation was then stopped for half the plants until they reached a water potential of ca. -0.4 to -0.6 MPa. All plants were then transferred to a growth chamber, where water deficit or well watered conditions were maintained. Ovule samples were collected at two stages: at the emergence of silks and 5 days after silk emergence. At each stage, ovules were collected from five zones of the ear (numbered 1 to 5 from bottom to top, ie. from the oldest to the youngest ovules). Three biological replicates were taken for each treatment*day*zone combination.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Kernel

SUBMITTER: Thierry Balliau

LAB HEAD: Elodie Marchadier

PROVIDER: PXD053569 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20110414_Ashenafi_Mariamawit_1_A01_61C1.mzXML	Mzxml
	20110414_Ashenafi_Mariamawit_1_A01_61C1.xml	Xml
	20110414_Ashenafi_Mariamawit_1_A02_61C2.mzXML	Mzxml
	20110414_Ashenafi_Mariamawit_1_A02_61C2.xml	Xml
	20110414_Ashenafi_Mariamawit_1_A03_61C3.mzXML	Mzxml

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Publications

A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries.

Balliau Thierry T Ashenafi Mariamawit M Blein-Nicolas Mélisande M Turc Olivier O Zivy Michel M Marchadier Elodie E

Biomolecules 20240930 10

Water deficit is a major cause of yield loss for maize (<i>Zea mays</i>), leading to ovary abortion when applied at flowering time. To help understand the mechanisms involved in this phenomenon, the proteome response to water deficit has been analysed in developing ovaries at the silk emergence stage and five days later. Differential analysis, abundance pattern clustering and co-expression networks were performed in order to draw a general picture of the proteome changes all along ovary developm ...[more]

PMID: 39456174

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