Dataset Information

Analysis of C. elegans DAF-16 phosphorylation in different genetic backgrounds.

ABSTRACT: C. elegans DAF-16/FOXO phosphorylation in different genetic backgrounds was analyzed by mass spectrometry of affinity purified HA-tagged DAF-16. Genetic backgrounds studied include par-1 hypomorphic mutants, PAR-1 knockdown using an auxin-inducible degron (AID), daf-2(e1370) and akt-1(ok525) mutants. PAR-1 is the C. elegans ortholog of MARK kinases; DAF-2 is the sole C. elegans insulin/IGF receptor, and AKT-1 is one of two AKT kinases in the nematode.

INSTRUMENT(S):

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Markus Hartl

LAB HEAD: Mario de Bono

PROVIDER: PXD053590 | Pride | 2025-12-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20231031_E2_NEO4_Artan_deBono_MPLGmbH_Academic_MPLID23213_s1_45p.raw	Raw
	20231031_E2_NEO4_Artan_deBono_MPLGmbH_Academic_MPLID23213_s2_45p.raw	Raw
	20231031_E2_NEO4_Artan_deBono_MPLGmbH_Academic_MPLID23213_s3_45p.raw	Raw
	20231031_E2_NEO4_Artan_deBono_MPLGmbH_Academic_MPLID23213_s4_45p.raw	Raw
	20231031_E2_NEO4_Artan_deBono_MPLGmbH_Academic_MPLID23213_s5_45p.raw	Raw

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Publications

Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins.

Artan Murat M Schoen Hanna H de Bono Mario M

Nature communications 20251211 1

Insulin/insulin-like growth factor signaling inhibits FOXO transcription factors to control development, homeostasis, and aging. Here, we use proximity labeling to identify proteins interacting with the C. elegans FOXO DAF-16. We show that in well-fed, unstressed animals harboring active insulin signaling, DAF-16 forms a complex with the PAR-1/MARK serine/threonine kinase, a key regulator of cell polarity. PAR-1 inhibits DAF-16 accumulation and promotes DAF-16 phosphorylation at S249, at a conse ...[more]

PMID: 41381452

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Project description:modENCODE_submission_3380 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: OP222(official name : OP222 genotype : unc119(ed3);wgIs222(daf-12:TY1 EGFP FLAG;unc119) outcross : 3 transgene : daf-12 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : Mihail Sarov ); Developmental Stage: L4; Genotype: unc119(ed3);wgIs222(daf-12:TY1 EGFP FLAG;unc119); Sex: Hermaphrodite; Transgene: daf-12; EXPERIMENTAL FACTORS: Developmental Stage L4; Target gene daf-12; Strain OP222(official name : OP222 genotype : unc119(ed3);wgIs222(daf-12:TY1 EGFP FLAG;unc119) outcross : 3 transgene : daf-12 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : Mihail Sarov ); temp (temperature) 20 degree celsius

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