The Valine-Arginine dipeptide repeat protein encoded by mammalian telomeric RNA appears highly expressed in mitosis and may repress global translation

ABSTRACT: Translation of mammalian telomeric G-rich RNA via the Repeat Associated non-AUG translation mechanism can produce two dipeptide repeat proteins: repeating valine-arginine (VR) and repeating glycine-leucine (GL). Their potentially toxic nature suggests that to be retained through evolution, one or both must play an important role in cells. Immunofluorescence staining revealed VR is in high abundance during mitosis and co-localizes with ribosomes and is highly abundant in the ventricular zone of the mouse cerebral cortex. To better understand the roles of VR, we sought to identify VR protein binding partners, we used affinity purification-mass spectrometry. Gene ontology enrichment analysis of VR binding partners revealed significant enrichment of ribosomal, mitotic, mRNA processing and DNA repair terms. Together, these data suggest a role for the VR dipeptide in mitotic progression and potentially in mRNA processing and translation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christine Mills  

LAB HEAD: Jack D. Griffith

PROVIDER: PXD054040 | Pride | 2026-05-25

REPOSITORIES: Pride