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Large-scale interactome screen for predicted transcription factors in fission yeast using IP-MS.

ABSTRACT: Large-scale interactome screen for 89 transcription factors (TFs) in fission yeast. TFs are tagged at the endogenous locus with a 3xFLAG affinity epitope. Cells cultured under optimal conditions (30°C, rich medium) were subjected to IP-MS at 150 mM an 500 mM NaCl.

INSTRUMENT(S):

ORGANISM(S): Schizosaccharomyces Pombe 927

SUBMITTER: Jan Seebacher

LAB HEAD: Marc Buehler

PROVIDER: PXD054070 | Pride | 2024-08-30

REPOSITORIES: Pride

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	L2_210514_vyt_2071_IP_S1.raw	Raw
	L2_210514_vyt_2071_IP_S2.raw	Raw
	L2_210514_vyt_2071_IP_S3.raw	Raw
	L2_210514_vyt_2071_IP_S4.raw	Raw
	L2_210514_vyt_2071_IP_S5.raw	Raw

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Publications

A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe Merle M Soneson Charlotte C Stadler Michael B MB Schwaiger Michaela M Suma Sreechakram Vishnu N VN Iesmantavicius Vytautas V Hess Daniel D Moreno Eliza Pandini Figueiredo EPF Braun Sigurd S Seebacher Jan J Smallwood Sebastien A SA Bühler Marc M

Molecular cell 20250226 7

Transcription factors (TFs) are key regulators of gene expression, yet many of their targets and modes of action remain unknown. In Schizosaccharomyces pombe, one-third of TFs are solely homology predicted, with few experimentally validated. We created a comprehensive library of 89 endogenously tagged S. pombe TFs, mapping their protein and chromatin interactions using immunoprecipitation-mass spectrometry and chromatin immunoprecipitation sequencing. Our study identified protein interactors for ...[more]

PMID: 40015273

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