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Mouse hippocampus 4D label free proteomic

ABSTRACT: Proteomic analysis of hippocampal tissues in control and surgery groups of mice was conducted to investigate the effects of surgery on mice at the protein expression level. The findings aim to provide insights into potential mechanisms underlying cognitive impairment following surgery.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Shuming Li

LAB HEAD: Xiaoping Gu

PROVIDER: PXD054165 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	CON1_Slot1-49_1_2635.d.zip	Other
	CON2_Slot1-50_1_2636.d.zip	Other
	CON3_Slot1-51_1_2637.d.zip	Other
	HA1_Slot1-46_1_2630.d.zip	Other
	HA2_Slot1-47_1_2631.d.zip	Other

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Publications

Microglia mediate memory dysfunction via excitatory synaptic elimination in a fracture surgery mouse model.

Li Shuming S Liu Huan H Lv Pin P Yao Yu Y Peng Liangyu L Xia Tianjiao T Yan Chao C Ma Zhengliang Z Chen Zhang-Peng ZP Zhao Chunjie C Gu Xiaoping X

Journal of neuroinflammation 20240916 1

Cognitive impairment is a common issue among human patients undergoing surgery, yet the neural mechanism causing this impairment remains unidentified. Surgical procedures often lead to glial cell activation and neuronal hypoexcitability, both of which are known to contribute to postoperative cognitive dysfunction (POCD). However, the role of neuron-glia crosstalk in the pathology of POCD is still unclear. Through integrated transcriptomics and proteomics analyses, we found that the complement ca ...[more]

PMID: 39285282

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Phosphoproteomic profiling of hippocampus from offspring rats exposed to prenatal stress

Project description:Prenatal stress (PS) could cause depression of offprsing. Here, relative quantitative phosphoproteomics of hippocampus of PS-S and CON offspring rats were performed using LC-MS/MS to confirm known pathways and identify new areas of depression. A total of 6,790 phosphopeptides, 9,817 phosphorylation sites and 2978 phosphoproteins were detected. Among the 2978 phosphoproteins, 1760 (59.09%) with more than two phosphorylated sites, ENSRNOP00000023460 protein has more than 117 phosphorylated sites, and the average distribution of modification sites per 100 amino acids was 2.97. There were 197 different phophpeptides, including 140 increased phosphopeptides and 57 decreased phosphopeptides in PS-S offspring rats when compared to the CON offspring rats. These differential phosphopeptides corresponded to 100 upregulated and 44 downregulated phosphoproteins respectively. GO enrichment analysis these different phosphoproteins in the first five enrichments of CC category, MF category and BP category were involved in a total of 35 different phosphoproteins and these phosphoproteins were mainly related to myelin, microtubule and synapse associated proteins. The first five enrichment of KEGG pathways were found to be involved in many essential biological pathways, and the first five pathways included amphetamine addiction, insulin secretion, cushing syndrome and circadian entrainment signaling pathway. And these first five pathways were related to nine proteins including Adcy9, Apc, Cacna1c, Camk2a, Camk2b, Camk2g, Ctnnd2, Grin2a, Stx1a. Further research is needed to understand the precise role of these pathways in the depressive-like behavior of offspring rats caused by PS.

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2019-06-24 | PXD010883 | Pride

Hippocampus-based mitochondrial respiratory function decline is responsible for perioperative neurocognitive disorders

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