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RBC Proteomics of different types of Thalassemia subjects

ABSTRACT: 10 patients with Hb E/ Beta Thalassemia with same genotype within 8 to 20 years of age and 5 healthy normal volunteer with age sex matched were selected for the RBC proteomics study.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Blood

DISEASE(S): Thalassemia

SUBMITTER: ANUPAM BASU

LAB HEAD: Anupam Basu

PROVIDER: PXD054385 | Pride | 2026-06-08

REPOSITORIES: Pride

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Publications

Multiomics analysis of red blood cells reveals thalassemia severity beyond globin gene mutations.

Mitra Nibedita N Bhattacharyya Upasana U Chowdhury Prosanto P Pal Arijit A M Korwar Arvind A Bhattacharjee Samsidhhi S Basu Anupam A

Blood advances 20260501 10

<h4>Abstract</h4>Hemoglobinopathies are the most common monogenic genetic disorders, primarily managed through blood transfusions or bone marrow transplantation. Clinical severity other than mutational effect is not well investigated and still unknown. This study aimed to identify dysregulated molecular pathways in red blood cells (RBCs) contributing to thalassemia severity. From a cohort of 285 patients with hemoglobinopathy, 10 age-matched individuals with identical compound heterozygous mutat ...[more]

PMID: 41616282

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Multiparametric characterization of red blood cells physiology after hypotonic dialysis based drug encapsulation process.

Project description:Red blood cells (RBC) can act as carriers for therapeutic agents and given their biocompatibility and long lifespan in the circulation, they can substantially improve the safety, pharmacokinetics, and pharmacodynamics of many drugs. Maintaining RBC integrity and lifespan is important for the efficacy of RBC as drug carrier and can be a complex challenge of drug encapsulation. We investigated the impact of drug encapsulation by hypotonic dialysis on RBC physiology and integrity. Several parameters were compared between processed RBC loaded with L-asparaginase (Ã¢â‚¬Å“eryaspaseÃ¢â‚¬ï¿½), processed RBC without drug and non-processed RBC. Processed RBC were less hydrated and displayed a reduction of intracellular content, relative to non-processed RBC. This reduction of intracellular content changed the RBC metabolomic profile, as indicated by the activation of the pentose phosphate pathway, but no impact on the global RBC proteomic profile was observed. We found that the encapsulation process caused moderate morphological changes and was accompanied by an increase of microparticles. Despite a decrease in their deformability, processed RBC were not mechanically retained in a spleen-mimicking device. Moreover, processed RBC had increased surface-to-volume ratio and osmotic resistance. Finally, the half-life of processed RBC was not significantly affected in a mouse model and our previous phase 1 clinical study showed that encapsulation of asparaginase in RBC prolonged its in vivo half-life compared to free form. Overall, our study demonstrated that encapsulation by hypotonic dialysis may affect certainÃ‚Â several characteristics of RBC, but does not significantly impact on the in vivo longevity of RBC or their drug carrier function.

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Ubiquitin C-terminal Extension (CxUB) DUB cleavage assay

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Nogo-B co-IP-MS experiment in mouse liver and pancreas

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