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Identification of cardiac myosin heavy chain post-translational modifications in hibernating grizzly bears

ABSTRACT: Mammal hibernation is a physiological process with unclear molecular mechanisms. Here, we aimed to investigate whether cardiac myosin heavy chain post-translational modifications would be affected by hibernation. For that, we used cardiac left ventricle specimens from active and hibernating adult grizzly bears. We then defined the nature and location of cardiac myosin heavy chain post-translational modifications using LC/MS.

INSTRUMENT(S): Orbitrap Ascend

ORGANISM(S): Ursus Arctos (brown Bear) (grizzly Bear)

TISSUE(S): Cardiac Muscle

SUBMITTER: Michael Wierer

LAB HEAD: Julien Ochala

PROVIDER: PXD054499 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears.

Van der Pijl Robbert J RJ Ma Weikang W Lewis Christopher T A CTA Haar Line L Buhl Amalie A Farman Gerrie P GP Rhodehamel Marcus M Jani Vivek P VP Nelson O Lynne OL Zhang Chengxin C Granzier Henk H Ochala Julien J

Molecular metabolism 20241216

<h4>Aim</h4>The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals.<h4>Methods</h4>Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics.<h4>Main findings</h4>Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are li ...[more]

PMID: 39694092

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Identification of phosphorylation and other post-translational modifications in the central C4-C5 domains of murine cardiac myosin binding protein C

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