Dataset Information

Phototropin from Marchantia polymorpha LC-MSMS

ABSTRACT: A blue-light receptor phototropin (phot) mediates chloroplast relocation responses. Upon receiving blue light, phot undergoes autophosphorylation via the serine/threonine kinase domain. The autophosphorylation levels of phot change in response to different blue-light intensities and temperature, and its level of autophosphorylation is associated with the induction of different types of chloroplast relocation responses. However, the phosphorylation sites of phot in the liverwort Marchantia polymorpha (Mpphot) is unknown. Here, we comprehensively surveyed serine and threonine residues as potential phosphorylation sites by using nano LC-MSMS and 23 phosphorylation sites and 18 candidates were identified.

INSTRUMENT(S):

ORGANISM(S): Marchantia Polymorpha

SUBMITTER: Minoru Noguchi

LAB HEAD: Yutaka Kodama

PROVIDER: PXD054673 | Pride | 2026-06-18

REPOSITORIES: Pride

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Publications

Phototropin switches between cis- and trans-autophosphorylation in light-induced chloroplast relocation in Marchantia polymorpha.

Noguchi Minoru M Noda Saki S Matsubayashi Yoshikatsu Y Kodama Yutaka Y

The Plant journal : for cell and molecular biology 20241203 1

In the accumulation response, chloroplasts move toward weak blue light (BL) to maximize photosynthetic efficiency; in the avoidance response, they move away from strong BL to reduce photodamage. The BL receptor kinase phototropin (phot) mediates these chloroplast relocation responses, and the chloroplast relocation response requires phot kinase activity. Upon receiving BL, phot undergoes autophosphorylation; however, the molecular mechanisms that regulate chloroplast relocation through phot auto ...[more]

PMID: 39625872

Similar Datasets

Project description:We have studied the transcriptional, metabolic and photo-physiological responses to light of different spectral quality in the marine diatom Phaeodactylum tricornutum through time-series studies of cultures exposed to equal doses of photosynthetically usable radiation of blue, green and red light. The experiments showed that short-term differences in gene expression and profiles are mainly light quality-dependent. Transcription of photosynthesis-associated nuclear genes was activated mainly through a light quality-independent mechanism likely to rely on chloroplast-to-nucleus signaling. In contrast, genes encoding proteins important for photoprotection and PSII repair were highly dependent on a blue light receptor-mediated signal. Changes in energy transfer efficiency by light-harvesting pigments were spectrally dependent; furthermore, a declining trend in photosynthetic efficiency was observed in red light. The combined results suggest that diatoms possess a light quality-dependent ability to activate photoprotection and efficient repair of photodamaged PSII. In spite of approximately equal numbers of PSII-absorbed quanta in blue, green and red light, the spectral quality of light is important for diatom responses to ambient light conditions. Continuous, axenic culturing of P. tricornutum was done as described in Nymark et al. (2009). The cultures were incubated at 15M-BM-0C under cool white fluorescent light (Philips TLD 36W/96) providing a scalar irradiance (EPAR) of 100 M-NM-<mol m-2 s-1 under continuous white light (CWL) conditions. Upon the onset of the experiment the cultures were synchronized by 48 h dark-treatment (D48). Thereafter the algae were exposed to blue light (BL), green light (GL) or red light (RL) provided by a waveband specific LED panel (SL3500, Photon Systems Instruments). The algae were exposed to 0.5 h, 6 h or 24 h of: 1) 230 M-NM-<mol m-2 s-1 of RL, 2) 100 M-NM-<mol m-2 s-1 of GL, 3) 50 M-NM-<mol m-2 s-1 of BL or 4) 100 M-NM-<mol m-2 s-1 of WL respectively. Three biological replicas for each of the treatments were harvested. Data for the white light exposure and 48h dark treatment is described in GSE42039, PMID: 23520530.

			Action	DRS
	Dark-Chymotrypsin.raw	Raw
	Dark-Chymotrypsin.txt	Txt
	Dark-inGel-e1.raw	Raw
	Dark-inGel-e1.txt	Txt
	Dark-inGel-e2.raw	Raw