Proteome4Pandemic Preparedness: Proteomics, an agnostic approach to identify virusesProteome4Pandemic Preparedness: Proteomics, an agnostic approach to identify viruses

ABSTRACT: Humans can be infected by a wide variety of virus species. To detect these different viruses, a data analysis approach for shotgun proteomic data was developed. For this proteome for pandemic preparedness (P4PP) pipeline, a database (P4PPv01) and a web application (P4PP) were constructed. The P4PP pipeline enables the identification of 1896 virus species from the 32 virus families in which at least one human-infectious virus is described. To evaluate the developed analysis pipeline 12 purchased heat inactivated supernatant of virus cultures (Helvetica Health Care Sàrl, Geneva, Switzerland), the supernatant of an in-house cultured Human Adenovirus 5 (ATCC VR-5) and Modified Vaccinia virus Ankara (ATCC VR1508). The purchased supernatant of the viruses cultures and Human Adenovirus 5 were tested undiluted. Vaccinia virus Ankara were tested, undiluted, 2.5 x and 5 x diluted supernatant.) were analyzed.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): West Nile Virus Human Adenovirus C Serotype 5 (hadv-5) (human Adenovirus 5) Modified Vaccinia Ankara Virus Influenza A Virus H3n2 Influenza B Virus Human Orthorubulavirus 4 Severe Acute Respiratory Syndrome Coronavirus 2 H1n1 Subtype Rhinovirus A Measles Virus Genotype B3 Human Parechovirus 3

TISSUE(S): Cell Culture

SUBMITTER: Armand Paauw  

LAB HEAD: Dr. ir. A.M.J. (Annette) Kliphuis

PROVIDER: PXD054958 | Pride | 2025-05-27

REPOSITORIES: Pride