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An integrative analysis of cell-specific transcriptomics and nuclear proteomics of sleep-deprived mouse cerebral cortex

ABSTRACT: Sleep regulation follows a homeostatic pattern. The mammalian cerebral cortex is the repository of homeostatic sleep drive and neurons and astrocytes of the cortex are principal responders of sleep need. The molecular mechanisms by which these two cell types respond to sleep loss are not yet clearly understood. By combining cell-type specific transcriptomics and nuclear proteomics we investigated how sleep loss affects the cellular composition and molecular profiles of these two cell types in a focused approach. The results indicate that sleep deprivation regulates gene expression and nuclear protein abundance in a cell-type-specific manner. Our integrated multi-omics analysis suggests that this distinction arises because neurons and astrocytes employ different gene regulatory strategies under accumulated sleep pressure. These findings provide a comprehensive view of the effects of sleep deprivation on gene regulation in neurons and astrocytes.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Pawan Jha

LAB HEAD: Akhilesh B. Reddy

PROVIDER: PXD055214 | Pride | 2025-05-30

REPOSITORIES: Pride

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			Action	DRS
	AstroNuclearGlobal_Sample_6_Run_1.raw	Raw
	AstroNuclearGlobal_Sample_6_Run_2.raw	Raw
	AstroNuclearGlobal_Sample_6_Run_3.raw	Raw
	Astrocyte_Nuclear_mzTab.mzTab.gz	Mztab
	Astrocyte_Nuclear_proteinGroups.txt	Txt

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