Proteomics

Dataset Information

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Comparative analysis of intra- and extracellular vesicles from cytotoxic CD8+ αβ T cells and γδ T cells

ABSTRACT: Although belonging to different branches of the immune system, cytotoxic CD8+ αβ T cells and γδ T cells utilize common cytolytic effectors including FasL, granzymes, perforin and granulysin. The effector proteins are stored in different subsets of lysosome-related effector vesicles (LREVs) and released to the immunological synapse upon target cell encounter. EVs of activated and expanded cytotoxic CD8+ αβ T cells or γδ T cells were enriched from culture supernatants by differential and ultracentrifugation and characterized by a comparative proteomic profiling with tandem mass tags (TMT10plex).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture

SUBMITTER: Andreas Tholey  

LAB HEAD: Andreas Tholey

PROVIDER: PXD055377 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20180731-Human_cRAP_LysC_Reviewed_Canonical_only.fasta Fasta
20220111-Exosome_TMT_120_1uL_R1-4_Combined.pdResult Other
20220111-Exosome_TMT_120_1uL_R1.raw Raw
20220111-Exosome_TMT_120_1uL_R2.raw Raw
20220111-Exosome_TMT_120_1uL_R3.raw Raw
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Publications

Comparative Analysis of Extracellular Vesicles from Cytotoxic CD8<sup>+</sup> αβ T Cells and γδ T Cells.

Griesel Lisa L   Kaleja Patrick P   Tholey Andreas A   Lettau Marcus M   Janssen Ottmar O  

Cells 20241021 20

<h4>Background</h4>Although belonging to different branches of the immune system, cytotoxic CD8<sup>+</sup> αβ T cells and γδ T cells utilize common cytolytic effectors including FasL, granzymes, perforin and granulysin. The effector proteins are stored in different subsets of lysosome-related effector vesicles (LREVs) and released to the immunological synapse upon target cell encounter. Notably, in activated cells, LREVs and potentially other vesicles are continuously produced and released as e  ...[more]

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