Dataset Information

Proteomics of isolated human cerebral vessels and whole tissue

ABSTRACT: Cerebral vasculature is critical in maintaining brain health, as its dysfunction is linked to various neurological illnesses including dementia and stroke. Proteomic studies are integral in understanding the biological mechanism underlying this dysfunction, identifying potential therapeutic targets and novel biomarkers of disease. However, translation of research findings into clinical practice relies on the validity and reproducibility of its methods. Thus, we have developed a novel protocol for the extraction of human cerebral vasculature which is highly compatible with downstream proteomic analyses. This protocol negates the need for bovine serum albumin, a ubiquitous reagent in vessel isolation protocols, yet an undesirable contaminant in proteomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Blanca Díaz Castro

LAB HEAD: Blanca Diaz-Castro

PROVIDER: PXD055837 | Pride | 2025-09-11

REPOSITORIES: Pride

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Publications

Molecular profiling of brain endothelial cell to astrocyte endfoot communication in mouse and human.

Hill Steven A SA Bravo-Ferrer Isabel I Čiulkinytė Austėja A Pérez Ramos Noelia N Rossetti Ilaria I Colvin Chiara C Beltran-Lobo Paula P Parra-Pérez Carlos C Emelianova Katie K Dando Owen O Geary Beth B Nirujogi Raja S RS Alessi Dario R DR Lee Do-Young DY Lee Youn-Bok YB Díaz Castro Blanca B

Nature communications 20251106 1

Our understanding of how the body communicates with the brain to coordinate their functions is remarkably limited. At the blood-brain barrier (BBB), brain endothelial cells (BECs) are ideally positioned to mediate signaling between blood and brain parenchyma via direct communication with astrocyte perivascular processes (endfeet). We develop a method to define the mouse in vivo astrocyte endfoot proteome, which in combination with BEC-specific RNA-seq, reveal BEC to astrocyte endfoot ligand-rece ...[more]

PMID: 41198665

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