Dataset Information

Establishment of Salivary Tissue-Organoid Biorepository

ABSTRACT: The salivary gland plays a crucial role in oral function and overall health by secreting saliva, yet salivary dysfunction due to aging, medications, autoimmune disorders, and cancer treatments poses significant challenges. We established the first diverse and clinically annotated salivary regenerative biobank at Mayo Clinic to study salivary gland stem/progenitor cells (SGSPCs). Optimization of cell isolation and progenitor assays revealed SGSPCs enriched within the CD24/EpCAM/CD49f+ and PSMA- phenotype of both submandibular and parotid glands, with clonal differentiation assays highlighting heterogeneity. Induction of PSMA/FOLH1 expression was associated with SGSPC differentiation. Utilizing label-free single-cell proteomics, we identified 819 unique proteins in SGSPC-enriched cells, including co-expressed cytokeratins, expressed in rare salivary ductal basal cells. Additionally, PRDX, a unique class of peroxiredoxin peroxidases enriched in SGSPCs demonstrated H2O2-dependent growth, suggesting a role in salivary homeostasis. These findings lay a solid foundation for studying SGSPCs and may lead to personalized regenerative medicine for salivary gland dysfunction.

INSTRUMENT(S): timsTOF SCP

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Akhilesh Pandey

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD055869 | Pride | 2025-05-26

REPOSITORIES: Pride

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Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1.

Aalam Syed Mohammed Musheer SMM Varela Ana Rita AR Khaderi Aalim A Mondesir Ronsard J RJ Mun Dong-Gi DG Ding Andrew A Lombaert Isabelle M A IMA Coppes Rob P RP Emperumal Chitra Priya CP Pandey Akhilesh A Janus Jeffrey R JR Kannan Nagarajan N

NPJ Regenerative medicine 20250521 1

The salivary gland (SG) is vital for oral function and overall health through secretion of saliva. However salivary dysfunction due to aging, medications, autoimmune disorders, and cancer treatments poses significant challenges. We established the first diverse and clinically annotated salivary regenerative biobank at Mayo Clinic to study salivary gland stem/progenitor cells (SGSPCs). Optimization of cell isolation and progenitor assays revealed SGSPCs enriched within the CD24/EpCAM/CD49f+ and P ...[more]

PMID: 40399315

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			Action	DRS
	PG_SCP.7z	Other
	PG_Set02.zip	Other
	Result.7z	Other
	SMG_SCP.7z	Other
	SMG_Set02.zip	Other