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Proteomic characterization of native and decellularized rat submandibular gland scaffolds prepared using the S3T1 protocol

ABSTRACT: Salivary gland hypofunction lacks effective regenerative treatment, and organ-specific decellularized extracellular matrix scaffolds represent a promising biomaterial platform for tissue engineering. In this study, rat submandibular glands were decellularized using a rapid S3T1 workflow consisting of freeze-thaw pretreatment, 1% SDS treatment for 3 h, and 0.25% trypsin-EDTA treatment for 1 h. Native submandibular glands and decellularized scaffolds were compared by data-independent acquisition LC-MS/MS to characterize protein composition and extracellular matrix preservation. The dataset was generated to identify retained matrisome components and to evaluate the proteomic features of the decellularized scaffold in comparison with native tissue. These data support the use of the decellularized submandibular gland scaffold as a bioactive platform for salivary gland tissue engineering.

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ORGANISM(S): Rattus Norvegicus (rat)

SUBMITTER: Jie Gao

LAB HEAD: Jie Gao

PROVIDER: PXD077315 | Pride | 2026-04-21

REPOSITORIES: Pride

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Project description:MyD88 is an important adaptor protein for signal transduction downstream of Toll-like receptors and TACI, receptors for regulation of innate immunity and B cell responses, respectively. The surfaces of oral mucosa are protected from infections by antimicrobial proteins and natural immunoglobulins that are constantly secreted in saliva, serving as principal innate immune defense in the oral cavity. Although MyD88-mediated signaling has a regulatory role in the intestinal mucosal immunity, its specific role in the oral cavity has been remained elusive. To identify the specific roles of MyD88-dependent signaling in gene expression profiles in salivary glands, we performed microarray analysis of the major salivary gland tissues (submandibular gland plus sublingual gland) from control (wild-type C57BL/6) mice and C57BL/6 background Myd88-null mice using Agilent Whole Mouse Genome Oligo Microarrays (8x60K, Design ID 028005). Total RNA was extracted from the major salivary gland tissues (submandibular gland plus sublingual gland) of wild-type and Myd88-null mice using using Trizol reagent (Life Technologies, Rockville, MD, USA) according to the manufacturer's instructions. 100 ng of all RNA samples was used as template to produce Cy3-labeled cRNA. The RNA samples were amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies, Palo Alto, USA) following the manufacturerM-bM-^@M-^Ys protocol. Cy3 labeled cRNAs were hybridized overnight (17 hours, 65M-BM-0C) to an Agilent Whole Mouse Genome Oligo Microarrays (8x60K, Design ID 028005) using AgilentM-bM-^@M-^Ys recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37M-BM-0C and a final wash step with acetonitrile for 30 sec at RT. Fluorescence signals of the hybridized Agilent Microarrays were detected using AgilentM-bM-^@M-^Ys Microarray Scanner System (G2505C, Agilent Technologies). The Agilent Feature Extraction Software (FES 10.7.3.1 ) was used to read out and process the microarray image files.

			Action	DRS
	FWMS20250734_Native1.raw	Raw
	FWMS20250734_Native2.raw	Raw
	FWMS20250734_Native3.raw	Raw
	FWMS20250734_S3T1-1.raw	Raw
	FWMS20250734_S3T1-2.raw	Raw

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Proteomic characterization of native and decellularized rat submandibular gland scaffolds prepared using the S3T1 protocol

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